There are a large number of biological seed treatments (e.g., fungi, amino acids, bacteria) marketed to growers under the premise that this practice will produce a positive ROI due to plant health promoting properties. Growers often purchase these products with the intention of maximizing yield to gain a competitive advantage. The effect of these products on yield, however, is largely undocumented. With the potential of low profit margins the effect of biological seed treatments on soybean yield and economic return is important to understand. Here, we will evaluate commonly-marketed biological seed treatment products across the U.S. This will help us identify environmental and soil factors where yield response to biological seed treatments is most likely to occur. We will test the bioligical seed treatments at two locations in Kentucky, allowing us to develop data locally. In addtition, we will compare results here with results from other states in the study to help us better understand if and when these seed treatments provide a yield benefit or a return on investment.