2025
Developing and releasing high-yielding soybean varieties/germplasm with climate-resilience and genetic diversity across maturity groups 00 to VIII
Category:
Sustainable Production
Keywords:
BiodiversityGeneticsGenomicsGlobal food security
Lead Principal Investigator:
Bo Zhang, Virginia Tech
Co-Principal Investigators:
Carrie Miranda, North Dakota State University
Caio Canella Vieira, University of Arkansas
Zenglu Li, University of Georgia
Feng Lin, University of Missouri
Ben Fallen, USDA/ARS-North Carolina State University
Rouf Mian, USDA/ARS-Ohio State University
+5 More
Project Code:
60065
Contributing Organization (Checkoff):
Institution Funded:
$343,250
Brief Project Summary:
Climate change has threatened soybean production and affected soybean growers’ profitability in the U.S. Increasing genetic yield potential and improving climate resilience using diverse soybean germplasm are important goals in soybean breeding and critical for sustainable soybean production in the U.S. The goal of this proposal is to develop and release climate-resilient soybean varieties across MG 00 to VIII.
Information And Results
Project Summary

Climate change has threatened soybean production and affected soybean growers’ profitability in the U.S. Increasing genetic yield potential and improving climate resilience using diverse soybean germplasm are important goals in soybean breeding and critical for sustainable soybean production in the U.S. With funding support from the United Soybean Board over the past several years, the team has successfully developed a strong pipeline of elite soybean materials with high-yield, climateresilience and diverse pedigrees and novel breeding technologies and tools to support breeding efforts. Based on previous discoveries and research foundation, seven scientists from six soybean-growing states covering MG 00 to VIII will work as a team in this proposal to achieve the following objectives: 1) evaluate and release high yielding soybean varieties with regional adaptation and climate resilience across MG 00 to VIII; 2) develop soybean germplasm with diverse genetic backgrounds for climate resilience; and 3) discover novel genes associated with climate resilience using emerging technologies and methodologies to support the breeding efforts. The outcomes generated from this proposal will include released varieties/germplasm and next-gen breeding materials with wide regional adaptation for commercial production, and novel genes associated with climate resilience. This work will benefit soybean growers in the U.S. by providing new soybean varieties adapted to regional growing conditions with resilience to climate changes. The project will also provide new and improved germplasm to the commercial and public breeders for use as parental stocks. Novel genes associated with climate resilience will benefit soybean researchers, enabling accelerated genetic gains.

Climate change is the leading factor that threatens agricultural production and food nutritive quality in the world by elevating the global temperature, disrupting regional rainfall patterns, accelerating biotic and abiotic stresses, increasing CO2 level, etc. (Raza et al., 2019; Saleem et al., 2001). Agricultural crop production is declining due to adverse and unstable environmental conditions (Rosenzerig et al., 2014). For example, a reduction of 86-92% of soybean yield by 2050 relative to 2013-2017 was projected using the data of yield, temperature and precipitation in the U.S. over 1951-2017 (Khanna, et al., 2021). In order to advance resilience agriculture, American Society of Agronomy, Crop Science Society and Soil Science Society of America have recommended irrigation management, water conservation, crop diversification, waste reduction, cover crop, and no-till farming to farmers. However, the simplest and most effective way to reduce the impact of climate changes on farms is to plant climate-resilient varieties. Increased genetic diversity and sustainable regional adaptability are essential for such varieties to perform well year after year. Current U.S. soybean cultivars have a very narrow genetic base to adapt to the stressful environments due to the changes in regional climatic conditions. However, worldwide soybean is a very diverse crop with adaptability to a wide range of climatic conditions that allow its cultivation in almost any country in the world. Nearly 22,000 soybean accessions in the USDA-Agricultural Research Service (USDA-ARS) Soybean Collection, Urbana, Illinois have captured most of the worldwide diversity of soybean in its exotic collections. The public soybean breeding programs represented in this team have worked for more than 15 years with partial funding from USB to develop genetically diverse breeding lines by crossing with some exotic lines from the USDA-ARS collection. As a result, our team will be able to release varieties with improved genetic diversity and resilience to the changing climatic conditions with just 1-3 years of research.

Project Objectives

The goal of this proposal is to develop and release climate-resilient soybean varieties across MG 00 to VIII.
Objectives:
Obj. 1: To evaluate and release high-yielding soybean varieties with regional adaptation and climate resilience across MG 00 to VIII.
Obj. 2: To develop soybean germplasm with diverse genetic background for climate resilience.
Obj. 3: To discover novel genes associated with climate resilience using emerging technologies and methodologies to support breeding efforts.

Project Deliverables

Project Metrics and Performance Measures (including Key Performance Indicators):
– Number of varieties/germplasm released from this project
– Seed companies show interest in our varieties to license them or evaluate them in their yield trials for future license
– Private and public soybean breeders/scientists agree to evaluate the climate-resilient germplasm released from this research and consider them as potential parents for their crosses to develop commercial high-yielding varieties with a diverse genetic base
– DNA markers developed for the climate-resilient related traits are used by soybean breeding programs for research and product development

Progress Of Work

Updated August 25, 2025:
For Obj. 1 - Evaluate and release high-yielding soybean varieties with regional adaptation and climate resilience across MG 0 to VIII
Across multiple U.S. regions, our team released and advanced soybean varieties that combine high yield with climate resilience and disease resistance. Virginia Tech released V19-1409RR, a high-yielding, Roundup Ready®, mid V variety that consistently outperformed trial averages in USDA and Virginia state tests. University of Missouri released two conventional MG IV lines, S20-2227 and S20-7117, both resistant to stem canker and yielding over 60 bu/ac across 40+ environments. University of Georgia released G19-13438 and G18-8335LL, which combine strong yield with nematode and disease resistance. USDA-ARS entered 48 elite lines into national regional trials; many performed above 100% of the test mean. University of Arkansas’s line R19C-1035 showed high yield and flood tolerance in multiple trials.

For Obj. 2 - Develop soybean germplasm with diverse genetic backgrounds for climate resilience
To strengthen long-term resilience, we are expanding the genetic base of U.S. soybean breeding programs by incorporating exotic and wild soybean traits. Virginia Tech is testing 65 advanced lines with at least 6.25% exotic ancestry, including wild soybean and elite Chinese germplasm. University of Missouri made crosses using heat- and drought-tolerant donor parents, with progeny under development in Costa Rica. University of Georgia evaluated 38 nematode-resistant lines in national trials and over 1,500 early-generation lines with resistance to biotic stresses. USDA-ARS advanced drought-tolerant lines (N21-0559, N21-0580) with high yields and protein content. University of Arkansas planted more than 18,000 genetically diverse progeny rows and initiated 195 new crosses—including combinations of drought × flood tolerance—to develop multi-stress-resilient soybeans. North Dakota State University developed three new populations by crossing locally adapted lines with SSNF (sustained symbiotic nitrogen fixation) germplasm. From 181 F6 lines, 12 were identified as well-adapted to ND conditions. These will undergo trait confirmation and field testing, supporting reduced fertilizer needs and improved performance under climate stress.

For Obj. 3 - Discover novel genes associated with climate resilience using emerging technologies and methodologies to support breeding efforts.
We are using cutting-edge tools to identify traits and genes that help soybeans survive extreme conditions. Virginia Tech used drone-mounted thermal cameras to monitor 387 soybean accessions for canopy temperature across three growth stages. Machine learning models accurately predicted real temperatures (R² = 0.97), enabling selection for heat resilience. University of Missouri is mapping photosynthetic traits in 250 accessions under heat and drought, while also accounting for genetic population structure. University of Georgia genotyped 170 advanced lines using 6,000 genetic markers, integrating yield and marker data into powerful predictive models. USDA-ARS advanced a yield-enhancing line derived from an exotic parent and evaluated it across 16 environments. University of Arkansas developed genomic models for flood tolerance and screened 400 soybean accessions predicted to be tolerant. These lines are now in multi-environment trials. Together, these innovations allow us to identify key resilience genes and accelerate breeding of soybean cultivars that will thrive in a changing climate.

View uploaded report Word file

Final Project Results

Updated July 25, 2025:

Benefit To Soybean Farmers

Plant breeding is the key solution to address the challenges of food security due to reduced crop productivity caused by climate change. Historically, plant breeders exploited wild relatives, selected beneficial traits, and cultivated many important crops through plant domestication (Purugganan and Fuller, 2009). Major milestones in plant breeding such as discovery of Mendel's Laws of inheritance and Green Revolution remarkably increased the yield of major crops to meet the rising food need (McCouch et al., 2013; Pingali, 2012). Nowadays, superior varieties with better climate resilience developed by soybean breeders through utilizing diverse genetic resources will tackle the future challenge of global crop productivity reduction.

Our team has committed to developing soybean varieties to meet growers’ needs. With previous funding supported by the USB and Multi-Regional Soybean Boards, we have built a strong and extensive breeding pipeline that has used exotic accessions, and/or wild soybeans, and produced many well-adapted high-yielding breeding lines. In 2023 and 2024, our team has released six varieties and germplasm with exotic pedigree (Table 1). Our team has also used innovative technologies and tools to discover genes and utilize them to improve yield, seed composition, climate resilience, and disease and pest resistance. In 2024, we were able to leverage these discoveries and adopt those breeding tools to develop varieties with climate resilience (Table 2).

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.