Achieving the objectives
• Several antibiotics were evaluated before proper treatments were devised to create endosymbiont-free aphid clones for testing
• Evaluation of the importance of endosymbionts to aphid virulence, or ability to overcome resistance showed that endosymbionts were not involved
Not achieving the objectives
• Some difficulties were encountered with maintaining live soybean aphid clone colonies. Throughout the study techniques at rearing and maintaining these clones were improved.
• Not all possible aphid biotype crosses have been made. So far those tested show a strong maternal influence for virulence, beyond that expected genetically.