Project Details:

Title:
Development of Soybean Cultivars with Increased Seed Oil Content While Maintaining Protein and Yield (Year 1 of 1420-632-6606)

Parent Project: This is the first year of this project.
Checkoff Organization:United Soybean Board
Categories:Seed composition, Breeding & genetics
Organization Project Code:1420-632-6606
Project Year:2014
Lead Principal Investigator:Leah McHale (The Ohio State University)
Co-Principal Investigators:
Pengyin Chen (University of Missouri)
Tom Clemente (University of Nebraska at Lincoln)
Rouf Mian (USDA/ARS-Ohio State University)
Keywords:

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

The versatility and use of soybean oil has continued to expand and with the forecasted transition to high-oleic soybean oil, demand is expected to further increase. We will evaluate the feasibility of increasing seed oil without effecting soybean yield or protein content by applying genetic, transgenic and mutational approaches. Seed oil and protein content are influenced by environmental factors, including field temperature. Thus, an important component in the project is to evaluate the environmental stability of each source of increased oil. Ultimately, we aim to develop soybeans with oil content greater than 20.5% (@ 13% moisture) while maintaining yield and protein content (>35% @ 13% moisture).

Project Objectives

1. Characterize the genes/loci involved in the increased oil trait from soybean plant introductions through genetic analysis of segregating populations
2. Discover new sources of increased oil/standard protein via biochemistry knowledge applied to the generation of transgenics and analysis of mutagenesis resources
3. Breed the increased oil trait into MGs II and V and high-oleic and normal-oleic types.
4. Backcross transgenic and mutant sources of increased oil into MGs II and V and high-oleic and normal-oleic types.
5. Assess the stability of the increased oil trait from transgenic sources in controlled environments differing for temperature.
6. Assess the stability of the increased oil trait from natural, mutant, and transgenic sources and the effect of increased oil on protein and yield in multiple northern and southern field environments.

Project Deliverables

1. Characterize the genes/loci involved in the increased oil trait from soybean plant introductions through genetic analysis of segregating populations
Linked markers or causal genes/alleles for high oil trait for four populations with unique sources of high oil.

Estimation of the effect of the major gene(s)/allele(s) on oil content for the four populations.

2. Discover new sources of increased oil/standard protein via biochemistry knowledge applied to the generation of transgenics and analysis of mutagenesis resources
Estimation of the effect of each mutation to seed oil and protein content in mutant lines (18).

Characterization of oil and protein content within transgenic lines (3 transgenes).

Estimation of the effect of the thin leaf mutant on oil and protein content.

3. Breed the increased oil trait into MGs II and V and high-oleic and normal-oleic types.
Crosses resulting in the most effective sources of high oil, >20.5% oil and > 35% protein (@13% moisture), in two potentially high yielding genetic backgrounds for both MG II and MG V.

Crosses resulting in the most effective sources of high oil, >20.5% oil and > 35% protein (@13% moisture), in high oleic types for both MG II and MG V.


4. Backcross transgenic and mutant sources of increased oil into MGs II and V and high-oleic and normal-oleic types.
BC2 lines with transgenic or mutational source of high oil (>20.5% @ 13% moisture) in both MG II and MG V genetic backgrounds.

5. Assess the stability of the increased oil trait from transgenic sources in controlled environments differing for temperature.
Characterization of the temperature stability of each of the three transgenes single and in combination.

6. Assess the stability of the increased oil trait from natural, mutant, and transgenic sources and the effect of increased oil on protein and yield in multiple northern and southern field environments.
Characterization of effect of different sources of the increased oil traits on yield and protein.

Characterization of stability of different sources of the increased oil trait in multiple environments.

Quantification of genotype by environment interactions.

Progress of Work

Final Project Results

Benefit to Soybean Farmers

Performance Metrics

Advancing knowledge:
Ultimately this project will result in an understanding of which genes and alleles contribute to oil and what effect the different sources of increased oil have on the protein and yield of the soybean. As seed oil content has been shown to be temperature dependent, importantly, this project will determine the environmental stability of each source of increased oil

Production of facilitating materials:
While three years is a limited time for cultivar development, this project will generate resources which will can lead to the rapid development of improved cultivars. Germplasm, genetic locations, linked markers, transgenic constructs and knowledge of key transgenes and stacks will all be generated from this project. These outputs facilitate the development of increased oil lines by industry breeders/researchers, thereby progressing the development of increased oil soybean cultivars which will add value to the US soybean.

Project Years

YearProject Title (each year)
2019Developing and Characterizing Soybean Germplasm with Increased Oil While Maintaining Protein and Yield (Year 3 of 1720-162-0111)
2018Developing and Characterizing Soybean Cultivars with Increased Oil While Maintaining Protein and Yield (Year 2 of 1720-162-0111)
2017Developing and Characterizing Soybean Cultivars with Increased Oil While Maintaining Protein and Yield (Year 1 of 1720-162-0111)
2016Development of Soybean Cultivars with Increased Seed Oil Content While Maintaining Protein and Yield (Year 3 of 1420-632-6606)
2015Development of Soybean Cultivars with Increased Seed Oil Content While Maintaining Protein and Yield (Year 2 of 1420-632-6606)
2014Development of Soybean Cultivars with Increased Seed Oil Content While Maintaining Protein and Yield (Year 1 of 1420-632-6606)