Historical Project Details: The role of GH3 genes in plant resistance against charcoal rot disease (2014)

2014

The role of GH3 genes in plant resistance against charcoal rot disease

Home

Contributor/Checkoff:

Kansas Soybean Commission

Category:

Sustainable Production

Keywords:

Crop protectionDiseaseField management

Parent Project:

The role of GH3 genes in plant resistance against charcoal rot disease

Lead Principal Investigator:

Bin Shuai, Wichita State University

Co-Principal Investigators:

Project Code:

Contributing Organization (Checkoff):

Kansas Soybean Commission

(n/a)

Institution Funded:

Wichita State University

(n/a)

Final Report

Brief Project Summary:

Charcoal rot is a plant disease caused by soil-borne fungus Macrophomina phaseolina. In Kansas, the crop most severely affected by charcoal rot is the soybean, especially in the southeast and east central regions of the state. The goal is to identify the full length cDNA sequences of 7 annotated Medicago GH3 genes using 5' - and 3' - RACE. Full length sequences have been obtained for two genes and partial sequences have been obtained for the other five. The difficulty was encountered during amplification of low abundant genes. Currently, experimental conditions and procedures are being optimized to achieve the goal.

Previous research successfully applied real-time quantitative PCR to...

Unique Keywords:
#charcoal rot, #soybean bioengineering, #soybean diseases, #soybean genomics

Information And Results

Final Project Results

Results indicated decreased damage from a whole plant health program and excellent yields. Some producers were not able to utilize the complete strip plot program but still had excellent yields. One individual had soybean gran yields of 75 bu/a across an entire 80 acre field.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.