2017
Development of a Ureide Tissue Test for Soybeans
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Contributor/Checkoff:
North Dakota Soybean Council
Category:
Sustainable Production
Keywords:
GeneticsGenomicsSeed quality
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
R Jay Goos, North Dakota State University
Co-Principal Investigators:
Jasper M. Teboh, North Dakota State University, Carrington Research Extension Center
Project Code:
Contributing Organization (Checkoff):
North Dakota Soybean Council
$6,950
Leveraged Funding (Non-Checkoff):
State Board of Ag Research and Education is contributing $6,950.00
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Institution Funded:
North Dakota State University
$6,950
Brief Project Summary:

Nitrogen flows from the roots of soybeans to the tops mostly as nitrate (from the soil solution) and compounds known as ureides (the export product of nitrogen fixation from the nodules). The concentration of ureides in the plant is directly related to the number and vigor of the nodules. In our laboratory, we have developed a simple and direct method of analyzing soybean tissues for ureide content. It is our hope that this test might someday be offered by soil and plant testing labs, but more field calibration data are needed. The purpose of this proposal is to conduct inoculation studies in the field, and relate the response (yield, protein content) to the ureide levels in the plants...

Unique Keywords:
#seed composition
Information And Results
Final Project Results

Update:
The final report was greater than 5 MB, so the executive summary was uploaded. The full final report is available by request at: rj.goos@ndsu.edu

View uploaded report Word file

The objective was to calibrate the ureide tissue test as an index of adequacy of N-fixation. Ureide-N levels in the plant axes (stems plus petioles) at flowering of less than 1000 ppm were associated with yield loss and protein loss due to inadequate N fixation. Levels greater than 1500 ppm were associated with adequate N fixation. We also conducted a calibration, comparing the numbers of Bradyrhizobium japonicum in the soil, to the response of soybeans to inoculation. Whenever numbers of B. japonicum in the soil were greater than 100 per gram, there was no response to inoculation.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.