2017
Rhg1, cqSCN Loci and Epigenetic Determinants of Resistance to Soybean Cyst Nematode (1720-172-0131)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
Improved Resistance to Soybean Cyst Nematode via Rhg1 and Other Soybean Loci (Year 1 of 1420-532-5643)
Lead Principal Investigator:
Andrew Bent, University of Wisconsin
Co-Principal Investigators:
Brian Diers, University of Illinois at Urbana-Champaign
Matthew Hudson, University of Illinois at Urbana-Champaign
+1 More
Project Code:
1720-172-0131
Contributing Organization (Checkoff):
United Soybean Board
$210,045
Institution Funded:
University of Wisconsin - Madison
$210,045
Brief Project Summary:

Unique Keywords:
#nematodes, #soybean cyst nematode, scn, scn resistance, rhg1 resistance
Information And Results
Final Project Results

Updated November 8, 2017:
• Research has been focused on identifying plants with crossover events in the regions where the SCN resistance QTL cqSCN-006 and cqSCN-007 are located. Overall 16 plants were identified, 5 with crossovers in cqSCN-006 and 11 close to cqSCN-007. The positions of the crossover events in the selected plants were mapped with markers and the selected plants are now being grown to maturity. Seed harvested from some of these plants will be grown in SCN tests to determine the position of the QTL relative to the crossover events.
• The chromosome walking for the SCN-cq006 and 007 loci is in final phases with small gaps to be closed. In addition to the large deletion located in the SCN-cq007 interval, a new candidate polymorphism has been identified in a gamma-SNAP protein, a key candidate gene in the SCN-cq006 interval. Although the polymorphism lies outside the coding region of the gene as annotated in the reference genome, the polymorphism may affect the coding sequence of an alternatively spliced mRNA expressed in the resistant genotype.
• Continued work with WCIC (former Monsanto soybean transformation facility, now UW-Madison facility), to generate transgenic CRISPR soybean lines for each candidate gene in the cqSCN-006 and cqSCN-007 intervals. For each of 17 candidate genes we have constructed and are now modifying plasmids for CRISPR mutagenesis.
• Ten plants that show a higher copy number than Fayette were selected along with five plants that show a lower copy number. Once the plants are mature, progeny from the plants will be tested for SCN resistance and any lines that show changes in resistance will be evaluated with other genetic methods to confirm their copy number.
• Regarding epigenetic status of Rhg1 locus: Several different experiments have been initiated: these include high resolution DNA methylation data for Rhg1 locus in 5 soybean varieties, studies of the Rhg1 chromatin status, and studies of the effect of hypo methylation on Rhg1 expression.

The knowledge that the number of copies of the gene Rhg1 may have bearing on the magnitude of SCN resistance within a variety will make a difference in the future of how varieties are bred for improved SCN resistance.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.