2019
Genomic Tools to Enable Trait Discovery and Deployment (1920-152-0131-A)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Heidi Kaeppler, University of Wisconsin - Madison
Co-Principal Investigators:
Shawn Kaeppler, University of Wisconsin - Madison
Mike Petersen, University of Wisconsin - Madison
+1 More
Project Code:
1920-152-0131-A
Contributing Organization (Checkoff):
United Soybean Board
$315,000
Institution Funded:
University of Wisconsin - Madison
$315,000
Brief Project Summary:

Unique Keywords:
#gene editing, crispr, tool development, genetics, #sustainability
Information And Results
Final Project Results

Updated December 2, 2021:
The overall goal of this research project was to conduct research to develop and refine soybean genetic engineering and gene editing systems that are efficient, robust, publicly available, and genotype-flexible for use in soybean functional genomics research and genetic improvement/breeding efforts. The impact of reaching the goals would aid in reducing the time and costs of soybean engineering/editing, enable transformation/editing of any preferred cultivar, and improve the accessibility and speed of genetic study and improvement of soybean in both the public and private sectors. Specific aims of this project included:
1. Creation of an updated and user-friendly Soybean Transformation/Editing portal on the website of the Wisconsin Crop Innovation Center transformation/editing service facility.
2. Construction and deployment of optimized soybean engineering/editing vectors.
3. Optimization of meristem-based transformation/editing protocols for multiple soybean cultivars.
4. Improvement in efficiency of non-Agrobacterium, meristem-based transformation systems for soybean.
5. Comparison of efficiency of various gene editing enzymes and systems.
6. Assessment of transformation and gene editing efficiency across multiple gene targets in soybean.
7. Investigation of nanoparticle-mediated editing/engineering systems for use in soybean that would enable transgene-free germline editing.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.