Project Details:

Breeding & Development of a Breadth of SB Cultivars

Parent Project: This is the first year of this project.
Checkoff Organization:Ohio Soybean Council
Categories:Breeding & genetics
Organization Project Code:18-R-06
Project Year:2018
Lead Principal Investigator:Leah McHale (The Ohio State University)
Co-Principal Investigators:

Contributing Organizations

Funding Institutions

Information and Results

Click a section heading to display its contents.

Project Summary

Our objectives aim to drive innovation through development of new traits for increased yield and specialty products. Development of food grade cultivars offer the opportunity to expand international niche markets. Development of high oleic cultivars meets a growing market demand. Improvement of pest and disease resistance in cultivars will increase yield. Training of the next generation of plant breeders aids in knowledge transfer. Ultimately, high yielding materials with superior added-value traits will be released as public or branded varieties for direct use by growers. Novel traits will be genetically characterized and materials will be released along with ‘breeder friendly’ molecular markers suitable for selecting the traits of interest. This will enable all released material to be easily integrated into private breeding programs; thereby, ultimately making the genetics widely available to Ohio farmers.

Project Objectives

1. Develop or work towards the development of food grade cultivars suitable for international markets:
(a) Tofu and soy milk,
(b) natto and soy sprouts,
(c) black seeded.

2. Integrate pest and pathogen resistance critical to adaptation to Ohio and/or quality of seed into food grade cultivars including resistance to:
(a) soybean aphid,
(b) soybean cyst nematode, and
(c) Phytophthora sojae.

3. Work towards the conversion of conventional breeding program to high oleic.

4. Training of the next generation of soybean breeders.

Project Deliverables

For the tofu and soy milk market breeding program, we will release one or more cultivars in Spring of 2017. Release will be determined by data from 2017 field trials as well as interest from producers and approval by the Crop Variety Release and Distribution Committee.

For our development of cultivars for the natto and soy sprout (small seeded) markets, in the winter of 2015 we sent 30 selected individuals from three cross combinations to Puerto Rico, making this seed available for limited tests in 2016. Initial tests were limited to two locations, with two reps at each. From this test, 11 lines were selected for seed size and yield. These lines are being grown in full yield trials in 2017 and will be evaluated for yield, agronomic traits, seed size and seed coat entirety. As we have received high levels of interest from producers and processors for natto lines, we will make a very small amount of seed for selected lines available for on-farm trials in 2018, with potential for early release in 2018.

For our development of black seeded soybean cultivars, over 200 F4 plants are being grown this summer. Preliminary seed color and yield testing will take place in 2018. Fully replicated yield trials will occur over two or three years, depending on performance and producer interest, starting in summer of 2019, with earliest release of cultivars in Fall of 2020.

First quarter (March 2018):
• Send seed to collaborators (Dorrance, Michel, Niblack) for greenhouse assays.
• Perform compositional analysis (protein and oil) on NIR & GC (~200).
• Make selections based on yield, disease, & seed composition data. Begin planting plans.

Second Quarter (June 2018):
• Propose releases to the Crop Variety Release and Distribution Committee.
• Send seed to collaborators for additional trial locations (regional uniform tests).
• Plant field tests (~10000) and seed increases, plant greenhouse crossing blocks.

Third Quarter (September 2018):
• Carry out crosses in greenhouse (>200).
• Collect DNA samples from F2 nursery lines, assay markers, make selections.
• Collect field notes. Rogue seed increases to maintain purity.

Fourth Quarter (December 2018):
• Harvest plots and crosses, analyze yield and agronomic data from harvest.
• Plant hybrid seed (>200) in greenhouse, send seed to winter nursery for advancement.

Progress of Work

Final Project Results

Updated April 30, 2020:
Protein and oil data was collected via NIR on 2-3 replicates of 2-3 locations of nearly 1000 breeding lines. Data for 2018 as well as multi-year summaries have been calculated. Based on this data as well prior reports on pest and disease resistance, we have prepared materials for release of three cultivars including two for the tofu and soy milk market. Of additional interest are seven natto cultivars that have undergone their second year of yield testing, with two lines exhibiting promising yield and seed size. Students have been making rapid progress, with one student (Stephanie Karhoff) having a manuscript on characterization of resistance to Phytophthora sojae recently accepted and preparing to defend her defense this semester.

Benefit to Soybean Farmers

Though commodity, non-GM soybean cultivars are not in high demand, the development and release of this material is critical for two reasons: (1) it feeds a small market of producers who grow public non-GM commodity soybean cultivars, (2) the release of cultivars and germplasm with excellent traits for Ohio adaptation provides sources of these traits to industry breeders. Release of our public cultivars and germplasm make traits for adaptation to Ohio accessible to all breeders.

Performance Metrics

Project Years