The most economically important soybean pathogen in the U.S. is the soybean cyst nematode (SCN), estimated to cause annual losses of more than $1.2 billion. The use of resistant cultivars remains the most effective means to mitigate the effect of SCN in soybean production. Persistent use of the same source of resistance exerted selection pressure on SCN populations, consequently, shifting their virulence. In other words, nematodes are becoming resistant to the resistance. SCN numbers in Ohio are rising. Our goal is to continue raising awareness and building understanding of such an important threat to Ohio soybean growers. We will provide Ohio soybean growers the opportunity to test soil samples for SCN (obj.1) and to determine the SCN virulence profile (obj.2). The first objective will help us know our numbers to more effectively select the best integrated SCN management strategies; the second objective will help us determine which resistance to SCN will be most effective in Ohio.