Crop diversification has the potential to promote soil health and increase farmer profitability. Further, researchers use several physical, chemical, and biological measurements to quantify changes in soil health in response to management. In this project we propose to perform an integrated analysis of soil health measurements within diversified cropping systems in Ohio. This proposal builds-upon efforts and expertise already in place at The Ohio State University. We will work with data from field experiments established in research stations, as well as data collected from on-farm surveys. These data include measurements of carbon pools, and microbial activity and diversity, including beneficial and pathogenic nematodes. Our analysis will these measurements to understand and/or predict soybean yield responses to practices that promote crop diversification. In addition, as part of this project, specific activities are planned to share research findings with the soybean industry in Ohio.