Gene editing and innovative mutation breeding approaches to develop 2nd generation improved soybean soluble carbohydrate composition
Sustainable Production
AgricultureCompositionGeneticsMacronutritional bundleSustainability
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Gunvant Patil, Texas Tech University
Co-Principal Investigators:
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Brief Project Summary:
The major goal of this project is to increase sucrose content to 10% and reduce RFOs content to boost soy nutritional value. Recently, we have discovered that mutations in sucrose biosynthesis genes (SuSuy15, PKF07) increased seed sucrose content from 4.3% to 8.0%, which is not commonly achieved before in mid or late maturity group (MG) soy. Thus, stacking mutant genes together and evaluating agronomic performance, we expect to increase sucrose content beyond 10% depending on the production area. Secondly, introgression of multi-genic trait into one elite line using conventional methods is highly challenging and therefore, we will apply gene-editing technology to create a genetic drive to develop high-sucrose (SuSy15, PKF07, INV) and low-RFOs (RS2) lines.
Information And Results
Project Summary

Project Objectives

Project Deliverables

Progress Of Work

Final Project Results

Sucrose is nutritionally useful and fully digested by monogastric animals, but RFOs are antinutritional and not digested in animal’s gut. Currently, commodity soybean seed contains 3.0-5.2% sucrose, 0.8 – 1.1% raffinose and 3.0–4.5% stachyose. This project aims to increase sucrose content over 10% and at the same time reduce RFOs boost the nutritional and economical value of soybean meal. Using innovative mutational breeding and gene-editing technology, we are stacking novel genes and applying gene-editing technology to boost sucrose content 10% or higher. In addition to novel genes, we are targeting well-characterized raffinose and stachyose synthase (RS/STS) and myo-inositol-3-phosphate synthase (MIPS) to efficiently generate lines with high-sucrose and low-RFOs. We have successfully identified several mutant lines with high sucrose content (7.3 – 10 %) using forward and reverse genetics. Importantly, we initiated gene-editing strategy to target known as well as novel genes and developed several CRISPR knockout mutant lines which will be characterized in FY2024.

Benefit To Soybean Farmers

Soybean meal protein serves as the primary source of metabolized energy in the diets of poultry and livestock. In addition to protein content, the desirability of higher soluble carbohydrate levels (specifically sucrose) and lower levels of raffinose and stachyose (members of the raffinose family oligosaccharides, RFOs) is a critical consideration for enhancing animal digestibility. These carbohydrate components are pivotal in determining the nutritional and economic value of soybean-based animal feed and food products. Sucrose is nutritionally beneficial and can be fully digested by monogastric animals, while RFOs are considered antinutritional as they remain undigested in the animal's gastrointestinal tract. At present, conventional soybean seeds typically contain sucrose levels ranging from 3.0% to 5.2%, with select high-sucrose variants containing up to 6.1% sucrose. Our research has led to the identification of multiple mutant soybean lines that exhibit sucrose content exceeding 8% in their seeds. These newfound sources hold the potential to expedite soybean breeding efforts aimed at enhancing the overall nutritional quality of soybean-based feed and food. Notably, we have successfully developed gene-edited lines using advanced CRISPR/Cas9 technology to combine high sucrose and low RFO traits, further advancing the potential for improved soybean varieties.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.