Project Details:

Genomic Tools to Enable Trait Discovery and Deployment (1920-152-0131-A)

Parent Project: This is the first year of this project.
Checkoff Organization:United Soybean Board
Categories:Sustainability, Breeding & genetics
Organization Project Code:1920-152-0131-A
Project Year:2019
Lead Principal Investigator:Heidi Kaeppler (University of Wisconsin - Madison)
Co-Principal Investigators:
Shawn Kaeppler (University of Wisconsin - Madison)
Mike Petersen (University of Wisconsin - Madison)
Keywords: gene editing, CRISPR, tool development, genetics

Contributing Organizations

Funding Institutions

Information and Results

Comprehensive project details are posted online for three-years only, and final reports indefinitely. For more information on this project please contact this state soybean organization.

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Final Project Results

Updated December 2, 2021:
The overall goal of this research project was to conduct research to develop and refine soybean genetic engineering and gene editing systems that are efficient, robust, publicly available, and genotype-flexible for use in soybean functional genomics research and genetic improvement/breeding efforts. The impact of reaching the goals would aid in reducing the time and costs of soybean engineering/editing, enable transformation/editing of any preferred cultivar, and improve the accessibility and speed of genetic study and improvement of soybean in both the public and private sectors. Specific aims of this project included:
1. Creation of an updated and user-friendly Soybean Transformation/Editing portal on the website of the Wisconsin Crop Innovation Center transformation/editing service facility.
2. Construction and deployment of optimized soybean engineering/editing vectors.
3. Optimization of meristem-based transformation/editing protocols for multiple soybean cultivars.
4. Improvement in efficiency of non-Agrobacterium, meristem-based transformation systems for soybean.
5. Comparison of efficiency of various gene editing enzymes and systems.
6. Assessment of transformation and gene editing efficiency across multiple gene targets in soybean.
7. Investigation of nanoparticle-mediated editing/engineering systems for use in soybean that would enable transgene-free germline editing.

Project Years