Enhancing Soybean Germplasm through Biotechnology
Sustainable Production
Lead Principal Investigator:
Tom Clemente, University of Nebraska at Lincoln
Co-Principal Investigators:
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Brief Project Summary:

This program targets the implementation of the tools of biotechnology as a means to introduce novel input and output traits into soybean. The derived transgenic events expressing the targeted trait(s) are characterized at the genetic level and subsequently phenotyped under greenhouse conditions. Lead transgenic events are then selected based on the data gathered from these initial genetic and phenotypic evaluations. Selected lead events per transgenic trait are then characterized under field environments at UNL’s Plant Biotechnology Field Facility for agronomics and stability of the trait(s) of interest.

The traits currently being researched and developed under this program include strategies...

Unique Keywords:
#breeding & genetics
Information And Results
Project Deliverables

The photosynthetic component of the program will include continuation of the field trials with selected lead events that carry the transgenic alleles shown to increase photosynthesis, in both standard leaf and lanceolate leaf genetic backgrounds, under both irrigated and non-irrigated environments. Moreover, the photosynthesis genes are being stacked with genes to increase carbon flux to oil during seed development, to investigate if this approach will translate to a soybean with higher oil, without impacting protein level. This program will also characterize novel transgenic soybean events that have reduced number of stomata on their leaves for drought tolerance. In addition, this program developed a single genetic element strategy that leads to the production of a soybean oil with saturated fatty acids, approximately, 25% coupled with oleic acid over 55%. Such an oil is a semisolid at room temperature and has uses in baking applications. Support of this program will enable us to conduct another field trial for more quantities for testing in end use applications. Reagents designed to create null mutations in three seed storage proteins are currently being introduced into soybean. Genotyping and subsequent monitoring of the protein quantity/quality of the derived soybean will be determined. In collaboration with Joe Lewis (Department of Entomology) and Bin Yu (School of Biological Sciences) soybean events, carrying interfering microRNAs towards aphids, will continue to be vetted for resistance to the insect. Lastly, in collaboration with Ed Cahoon, in Biochemistry at UNL, we are employing synthetic biology approaches to create multi-gene stacks for the development of a soybean for aquaculture.

Final Project Results

Updated February 12, 2021:

View uploaded report PDF file

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.