2015
Characterization and enhancement of soybean genetic resources for soilborne disease resistance
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Contributor/Checkoff:
North Central Soybean Research Program
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
James Kurle, University of Minnesota
Co-Principal Investigators:
Teresa Hughes, Purdue University
Jianxin Ma, Purdue University
Katy M Rainey, Purdue University
James Orf, University of Minnesota
Nevin Young, University of Minnesota
+4 More
Project Code:
Contributing Organization (Checkoff):
North Central Soybean Research Program
(n/a)
Institution Funded:
University of Minnesota
(n/a)
Brief Project Summary:

Soilborne diseases caused by various oomycete and fungal pathogens have been a major limitation to soybean production. Each year the U.S. soybean crop is attacked by several pathogens that cause estimated annual losses valued in the billions of dollars. Among these pathogens, Phytophthora sojae, Pythium ultimum, Pythium irregular, and Fusarium graminearum are the major culprits, particularly, in the North Cental Region.

The primary goals of this project are to characterize, identify, and/or isolate novel genes/QTLs conferring resistance or partial resistance to P. sojae, P. ultimum P. irregulare, and F. graminearum, and to deploy the new sources of resistance towards effective disease...

Unique Keywords:
#breeding & genetics, #seedling diseases, #soybean genetic mapping, #soybean germplasm screening
Information And Results
Final Project Results

During this project year tests of multiple methods of inoculation with F. graminearum were conducted to validate the methodology, effectiveness, and consistency of the method used to conduct screening for resistance to seed, seedling, and root rot caused by F. graminearum. Based on these tests, the rolled towel assay and rice inoculum method were selected as the most reliable screening methods for assessing root rot and root development.
Utilizing these methods, rolled towel and rice inoculum assay, we completed phenotyping and association mapping of results obtained from multiple phenotyping assays of 280 ancestral lines for resistance or partial resistance to P. sojae and F. graminearum.
Association mapping performed on results obtained in P. sojae and F. graminearum phenotyping indicating the location of markers for partial resistance to F. graminearum. In 280 ancestral lines two categories of marker were identified that indicated resistance to F. graminearum, one marker located on chromosome 8 indicating resistance to root necrosis and 10 markers distributed on multiple chromosomes associated with resistance to loss of root mass. These markers may also be good targets for genomic selection for root rot resistance. Association mapping of phenotypic data for P. sojae partial resistance did not detect significant marker- trait associations for resistance to P. sojae although phenotyping indicated that 280 lines differed widely in response to P. sojae inoculation. To examine this phenomenon more closely we have elected to examine the results of biparental crosses between cultivars exhibiting a wide divergence of resistance to P. sojae inoculation. For this reason we completed six crosses of lines with divergent resistance characteristics, resistant and susceptible, for use in biparental mapping of resistance and partial resistance to P. sojae.
First run consisting of one rep of evaluation of 280 lines for resistance or partial resistance to P. ultimum is underway.
Pinpointed location of RpsUN1 and RpsUN2, identified RpsUN2 candidate gene, and began haplotype analysis to identify SNP markers associated with RpsUN1 and RpsUN2. Introgressed RpsUN1 and RpsUN2 into prebreeding lines and elite breeding lines.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.