Project Details:

Engineered resistance to soybean cyst nematode via induced gene silencing (RNAi)

Parent Project: This is the first year of this project.
Checkoff Organization:North Central Soybean Research Program
Categories:Nematodes, Breeding & genetics
Organization Project Code:
Project Year:2012
Lead Principal Investigator:Harold Trick (Kansas State University)
Co-Principal Investigators:
Jiarui Li (Kansas State University)
Tim C. Todd (Kansas State University)
John Finer (The Ohio State University)
Lila Vodkin (University of Illinois at Urbana-Champaign)
Jack Widholm (University of Illinois-Carbondale)
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Keywords: Soybean Cyst Nematode - Genetic Resistance, Virus-inducted Gene Silencing

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

The primary goal of this research project is to establish a new set of biotech traits that have durable resistance to soybean cyst nematode (SCN). Turning off genes by a process known as RNA interference (RNAi) has tremendous potential as a new strategy to increase nematode resistance. Past research with other nematode species have demonstrated the scientific merit of the technique. This project will investigate the opportunities to insert target gene sequences in the SCN that will provide durable genetic materials that will be lethal to SCN populations.

Project Objectives

1. Engineer stable transgenic soybean plants with traits that can silence specific nematode genes.
2. Evaluate transgenic lines with SCN bioassays to confirm the effectiveness of the level of SCN resistance.
3. Examine the durability of the transgenic traits on single and diverse populations of SCN.

Project Deliverables

Progress of Work

Final Project Results

Transformation of soybean cultures with the six RNAi vectors have begun at the four institutions and are in the early stages of transformation. Bombardments have been performed on five of the six vectors. Although most of these experiments were started towards the end of the quarter, we have identified 23 events from three of the six vectors. Five of these have been confirmed via PCR and the other 17 were still too small to be sampled. The confirmed events are now being regenerated into plants. The other bombardments are still under selection.

Benefit to Soybean Farmers

Performance Metrics

Project Years