2012
Engineered resistance to soybean cyst nematode via induced gene silencing (RNAi)
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Contributor/Checkoff:
North Central Soybean Research Program
Category:
Sustainable Production
Keywords:
NematodePest
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Harold Trick, Kansas State University
Co-Principal Investigators:
Jiarui Li, Kansas State University
Tim C. Todd, Kansas State University
John Finer, The Ohio State University
Lila Vodkin, University of Illinois at Urbana-Champaign
Jack Widholm, University of Illinois-Carbondale
+4 More
Project Code:
Contributing Organization (Checkoff):
North Central Soybean Research Program
(n/a)
Institution Funded:
Kansas State University
(n/a)
Brief Project Summary:

The primary goal of this research project is to establish a new set of biotech traits that have durable resistance to soybean cyst nematode (SCN). Turning off genes by a process known as RNA interference (RNAi) has tremendous potential as a new strategy to increase nematode resistance. Past research with other nematode species have demonstrated the scientific merit of the technique. This project will investigate the opportunities to insert target gene sequences in the SCN that will provide durable genetic materials that will be lethal to SCN populations.

Unique Keywords:
#nematodes, #soybean cyst nematode - genetic resistance, #virus-inducted gene silencing
Information And Results
Final Project Results

Transformation of soybean cultures with the six RNAi vectors have begun at the four institutions and are in the early stages of transformation. Bombardments have been performed on five of the six vectors. Although most of these experiments were started towards the end of the quarter, we have identified 23 events from three of the six vectors. Five of these have been confirmed via PCR and the other 17 were still too small to be sampled. The confirmed events are now being regenerated into plants. The other bombardments are still under selection.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.