Project Details:

Specific and Efficient Suppression of Soybean Cyst Nematode in Soybean using Artificial Micro RNA Strategy (Year 1 of 2284)

Parent Project: This is the first year of this project.
Checkoff Organization:United Soybean Board
Categories:Nematodes, Breeding & genetics
Organization Project Code:2284
Project Year:2012
Lead Principal Investigator:Harold Trick (Kansas State University)
Co-Principal Investigators:

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

RNA interference (RNAi) refers to the silencing of gene expression through the use of sequence-specific, homologous RNA olecules. This phenomenon was first demonstrated in a free-living nematode Caenorhabditis elegans. RNAi strategy has been successfully demonstrated to control plant-parasitic nematodes. However, it is possible that the current RNAi vectors can silence unintended genes, even in the soybean plant itself. To solve this "off-target" problem, we will construct a different class of vectors called artificial micro RNA (amiRNA) vectors, which can lead to more specific gene silencing effects.

Project Objectives

1. Construct a different set of vectors called artificial micro NRA (amiNRA) vector.
2. Use vectors for specific gene silencing effects.

Project Deliverables

Progress of Work

Final Project Results

Benefit to Soybean Farmers

Performance Metrics

Soybean plants with engineered roots expressing amiRNA sequences will be used in greenhouse SCN bioassays and be compared to transgenic plants produced in FY2011 as well as non-transgenic control plants. The amount of nematode cysts and eggs will be scored and statistic analysis will be performed on these data.
After effective 21-nt siRNAs are identified by composite plant bioassay, we will continue hairy root bioassays by combining several effective 21-nt siRNAs in one amRNAi vector.
By the end of the FY2012, we will identify the effective amiRNA vectors with composite plant bioassay. Stable transgenic soybean lines containing these vectors will be initiated at the end of the first year and continue into FY2013.

Project Years