2012
Genetic Evaluation and Development of Germplasm Resistant to Multi-Nematodes in Group III, IV, and V Soybeans (Year 1 of 2501)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Grover Shannon, University of Missouri
Co-Principal Investigators:
Bob Robbins, University of Arkansas
H Roger Boerma, University of Georgia
+1 More
Project Code:
2501
Contributing Organization (Checkoff):
United Soybean Board
(n/a)
Institution Funded:
University of Missouri
(n/a)
Brief Project Summary:

Unique Keywords:
#nematodes, #soybean breeding - disease resistance, #soybean cyst nematode - genetic resistance
Information And Results
Final Project Results

Achieving the objectives:
• Screened over 600 soybean PIs for resistance to six SCN HG types. Identified 44 PIs, including unreported genotypes, showing high resistance to single- or multiple-SCN HG types.
• Completed phenotyping the 44 PIs for resistance to RKN and RN. Dual resistances of SNC and RKN was found for PIs 4004198B, P417091, 438489B, 468915, 494182, 507354, 507471, 548349, and 567516C. Seven of these lines PIs 404198B, 417091, 438489B, 468915, 507354, 507471, and 567516C have resistance to RN, as well, making them resistant to all 3 nematodes.
• Four mapping populations (Magellan x 438489B, Magellan x PI437690, Magellan x PI404198B) are being utilized for QTL mapping of multiple nematode resistances. Four QTL were identified in the 438489B populations- on Chromosomes 18, 8, 11, and 10.
• The genome wide association study (GWAS), using the 50,000 SNPs dataset developed by Drs. Cregan and Hyten, was completed using over 500 PIs from MG III-V evaluated for SCN HG type 0. In this study, six new loci were identified.
• Marker-assisted backcrossing (MABC) to develop near-isogenic lines (NILs) was progressed. Several BC4F2 crosses were made to develop NILS to use for identifying novel QTL regions on chromosomes 10 and 18.
• Fine-mapping of novel QTL on chromosome 10 with RILS of Magellan
• Fine-mapping of novel QTL O on Chr. 10 using 315 recombinant inbred lines (RIL) of the Magellan x PI 567516C population was completed. SSR markers associated with the QTL were developed, as were six additional SSR markers to be used for fine-mapping. To date the regions has been narrowed down 300 kb.
• Developed several productive lines with resistance to multiple nematode species. Twenty six advance breeding lines are being tested in yield trial in Missouri. All of the lines to PI437654 resistance. Two soybean varieties, S11-20356 and S11-20124, both with moderate resistance to SCN races 1, 2, 3, 5, and 14, RKN and RN were released in 2014. Both releases are also tolerant to salt and have frogeye leaf spot resistance.
• Other lines are being developed to add unique the SCN resistant alleles from PI 438489B and PI 567516c to elite lines with resistance to multiple nematode species in conventional and Roundup Ready backgrounds.

Not achieving objectives:
• None at this time.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.