Project Details:

Title:
Enhancement of soybean through genetic engineering

Parent Project: Enhancement of soybean through genetic engineering
Checkoff Organization:Kansas Soybean Commission
Categories:Breeding & genetics, Soybean diseases
Organization Project Code:
Project Year:2013
Lead Principal Investigator:Harold Trick (Kansas State University)
Co-Principal Investigators:
William Schapaugh (Kansas State University)
Tim C. Todd (Kansas State University)
Keywords: Genetically Engineered Soybean, Soybean Bioengineering, Virus-induced Gene Silencing

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

Decreasing yield loss and increasing the value of soybeans is part of KSU's mission to improve Kansas agriculture. This project takes a genetic engineering approach to this mission allowing the utilization of traits outside the scope of conventional breeding.

Project Objectives

1. Field test transgenic lines with increased soybean cyst nematode (SCN) resistance.
2. Enhance SCN resistance in transgenic soybean by modifying current RNAi strategies.
3. Test the effectiveness of RNAi for root knot nematode resistance using RKN genes homologous to effective SCN genes.
4. Continue to produce and evaluate genetically engineered soybeans for increased fungal resistance.

Project Deliverables

Progress of Work

Final Project Results

To increase antimicrobial resistance, we have synthesized three defensing-like genes. These gene sequences and have cloned, expression vectors were made and soybean transformations have been initialized. We anticipate testing the efficacy of these genes on a wide variety of soybean pathogens in and the near future. Some of the stable lines generated appear to be losing expression of the transgene and a result of transgene silencing. Field testing of transgenic lines are on hold until we can address this issue.

Benefit to Soybean Farmers

Performance Metrics

Project Years