Project Details:

Enhancement of soybean through genetic engineering

Parent Project: Enhancement of soybean through genetic engineering
Checkoff Organization:Kansas Soybean Commission
Categories:Breeding & genetics, Soybean diseases
Organization Project Code:1514
Project Year:2015
Lead Principal Investigator:Harold Trick (Kansas State University)
Co-Principal Investigators:
William Schapaugh (Kansas State University)
Tim C. Todd (Kansas State University)
Keywords: Genetically Engineered Soybean, RNAi Strategies, Soybean Bioengineering, Soybean Cyst Nematode (SCN), Trans-gene Silencing, Virus-induced Gene Silencing

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

Decreasing yield loss and increasing the value of soybeans is part of KSU's mission to improve Kansas' Agriculture. Our proposal is taking a genetic engineering approach to this mission allowing us to utilize traits outside the scope of conventional breeding.

Fungal pathogens and parasitic nematodes are important, persistent problems that cause large economic losses across the Midwest. For example, the total estimated loss for the US in 2010 due to SCN was 118 million bushels or $1.25 billion. Root knot nematodes are also a major factor for soybean yield loss in the southern US and have the potential to become a problem for Kansas producers.

Charcoal rot is the major fungal disease in the state of Kansas and losses in 2002 were estimated at 9%. Phytophthora root rot and Fusarium virguliforme (Sudden Death Syndrome, SDS) are other fungal pests that are beginning to make their presence in Kansas (SDS was at record levels in the 2004 growing season). It is timely to find methods to efficiently control to these pathogens as there is little or no natural sources of resistance found in our germplasm. Novel approaches such as using antimicrobial peptides have merit and should be explored. Finding transgenic solutions to Soybean Diseases would complement the efforts of the conventional breeding program by adding additional sources of resistance.

Project Objectives

1. Enhance Soybean cyst nematode (SCN) resistance in transgenic soybean by modifying current silencing strategies.
2. Test the effectiveness of gene silencing constructions for root knot nematode resistance using RKN genes homologous to effective SCN genes.
3. Develop transgenic approaches for increased fungal resistance with emphasis on SDS resistance.

Project Deliverables

Progress of Work

Final Project Results


View uploaded report PDF file

When taking Y25 E13P1 and P3 events as examples, we found that different plants from a single event have very varied expression levels according to the reporter, especially for T1 generation seeds which is segregating. As RT-qPCR working on the reporter, it will be the first guide to select high expression transgenic plants for offspring seeds of other transgenic lines later. So far, it is likely that stable transgenic lines Y25E13, Prp17 01-03P6 and J23 E4P1 have significant affects on SCN development, and could be one of good resources for SCN resistance. Other potential lines from initial screen test are under the way, and several next generations of seeds were produced too. The lines with significant SCN reduction will be tested again for replications.

Benefit to Soybean Farmers

Performance Metrics

Project Years