2012
Screening for genetic resistance against soybean viruses
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Contributor/Checkoff:
North Central Soybean Research Program
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
Screening for genetic resistance against soybean viruses
Lead Principal Investigator:
John Hill, Iowa State University
Co-Principal Investigators:
Steve Whitham, Iowa State University
Reza Hajimorad, University of Tennessee-Institute of Agriculture
Craig Grau, University of Wisconsin
+2 More
Project Code:
Contributing Organization (Checkoff):
North Central Soybean Research Program
(n/a)
Institution Funded:
Iowa State University
(n/a)
Brief Project Summary:

Previous research has found that the control of the disease caused by bean pod mottle virus (BPMV) could not be achieved through management of the virus vector, the bean leaf beetle. Therefore, the most practical and reliable method for disease control is disease resistance. However, adequate levels of resistance have not been available. Hill and Grau have developed a novel seed-based assay to measure the amount of virus in a seed to identify BPMV field tolerance. The method involves an engineered BPMV isolate that contains a gene for herbicide resistance. When soybean plants are inoculated with the engineered BPMV isolate, one can easily and rapidly assay for BPMV resistance. The goal of...

Unique Keywords:
#alfalfa mosaic virus (amv), #bean pod mottle virus (bpmv), #breeding & genetics, #soybean viruses
Information And Results
Final Project Results

Bean Pod Mottle Virus: (BPMV)
• Evaluated several hundred recombinant inbred lines for tolerance/resistance to BPMV using BPMV engineered to contain a gene herbicide resistance. Identified numerous (ca. 90) lines that showed resistance/tolerance to BPMV

• Selected a subset of lines using criteria of seed quality, yield loss, and symptom severity. Severity scale used is 0 (no symptoms) to 3 (severe symptoms). Average yield loss scale 1 – 31%, scale 2 = 39%, scale 3 = 45%. Some lines showed 0% yield loss.

• Some lines may exhibit complete resistance to BPMV

• Seed from lines are being assayed by quantitative ELISA technique developed to identify tolerance/resistance. Assays are complete. Data analysis is in progress

Alfalfa Mosaic Virus (AMV)
• Produced antiserum to AMV using a novel approach expressing part of AMV in a bacterium and using for antibody production. Antibodies are effective for detection of a large number of AMV strains.

• Identified sampling time for AMV detection relative to soybean leaf position and AMV inoculation time. Data suggest leaflet collections should be made pre-bloom.

• Seed-borne nature of AMV along with symptomless AMV isolates suggest conclusions based upon soybean symptom expression regarding susceptibility and potential for seed transmission may be erroneous.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.