2013
Increased profitability for South Dakota soybean growers and breeders using genes and germplasm for enhanced tolerance to environmental stress
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Contributor/Checkoff:
South Dakota Soybean Research and Promotion Council
Category:
Sustainable Production
Keywords:
Biotic stressCrop protectionField management Pest
Parent Project:
Increase profitability for South Dakota soybean growers and breeders using genes for enhanced tolerance to environmental stress
Lead Principal Investigator:
David Clay, South Dakota State University
Co-Principal Investigators:
Wanlong Li, South Dakota State University
Paul Rushton, South Dakota State University
+1 More
Project Code:
Contributing Organization (Checkoff):
South Dakota Soybean Research and Promotion Council
(n/a)
Institution Funded:
South Dakota State University
(n/a)
Brief Project Summary:

The goal of this project involves identifying and isolating genes, or germplasm, from cultivated and wild soybean that can be used to improve yield, seed quality, pest resistance, nutrient and water use efficiency in soybean plants. The project provides for innovative approaches to solve practical problems facing soybean producers in South Dakota.

Unique Keywords:
#insects and pests, #soybean aphid - genetic resistance, #soybean cyst nematode - genetic resistance, #soybean drought tolerance, #soybean gene expression, #soybean gene mapping
Information And Results
Final Project Results

Summary of Outputs/Accomplishments: Crop yields in the Northern Great Plains are limited by a range of stress factors associated with water availability. Across the state, one or two summer rains represent the difference between a drought and a bountiful season. The project has been very busy and produced many promising results. Some are listed here:
• 209 lines of soybeans with genetic resources from wild-soybean obtained among which roughly 30% have a high degree of tolerance against IDC.
• Specific pathways and roughly 2000 target genes identified for further analysis and genetic targeting.
• Proteomics techniques established to study soybean leaf and seed proteins including the identification of specific proteins associated with drought stress in the leaves.
• Identification and characterization of soybean signaling proteins (GmMAPKs).
• Ascorbate-glutathione pathways identified as a biomarker for drought tolerance in soybean
• Genes associated with nitrogen fixation and transport with potential for manipulation to enhance N availability during drought stress have been identified.
• Microbiome of soybean root surface using SD soils has been established to identify beneficial microbes promoting growth under stress
• Genes and genetic material with altered leaf architecture and stem hair development towards enhanced yields and insect/stress resistance.
• Accumulation of the phytoalexin coumestrol under water stress has been identified as one of the most dramatic changes in metabolites.
• Promoter analyses have identified both known and novel switches that turn on drought-responsive genes.
• Metabolomics, proteomics, and transcriptomics reveals that new synthesis of amino acids occurs. These act to protect the plant from dehydration.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.