This project was initiated preceding the incorporation and standardization of KPIs. The progress of objectives are as follows:

Achieving Objectives:
• Three single copy and two multi-copy founder lines were generated and crossed into Magellan, Williams, and Jack cultivars for greater utilization. The position of the T-DNA insert sites in each of the three lines with a single copy has been determined. This work involved using low-coverage Next Generation Sequencing (NGS) and custom designed software. The software was developed by USDA and is available to researchers.
• The initial test pEXCH vectors for biolistic and agro-bacterium transformation have been completed. The targeting (RCME) process has yielded 10 positive plants. Site specific targeting still needs to be validated.
• The Fad2-1 and Fad3 RNAi suppression transgenes and another gene designed to modify the beta carotene pathway have been cloned and testing is underway. The group is working with another USB funded project (1420-532-5604) to test disease resistance genes that were shown to be important using VIGS. This platform will allow optimal and consistent expression.

Not meeting Objectives:
• No issues at this time.