Project Details:

Modifying Soluble Carbohydrates in Soybean Seed for Enhanced Nutritional Energy Meal (Year 1 of 1720-152-0101)

Parent Project: This is the first year of this project.
Checkoff Organization:United Soybean Board
Categories:Seed composition, Breeding & genetics
Organization Project Code: 1720-152-0101
Project Year:2017
Lead Principal Investigator:Katy M Rainey (Purdue University)
Co-Principal Investigators:
John Brake (North Carolina State University)
Aaron Lorenz (University of Minnesota)
Pengyin Chen (University of Missouri)
Henry Nguyen (University of Missouri)
Grover Shannon (University of Missouri)
Kristin Bilyeu (USDA-ARS)
Karen Hudson (USDA-ARS-Purdue University)
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Keywords: carbohydrate, low oligosaccharides, RFO, sucrose, RS genes, germplasm

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

Modified soluble carbohydrate composition is the most promising meal trait due to potential value to the animal industry and lack of negative agronomic impacts. To develop this value-added market, varieties and germplasm need to be developed, and economic targets must be defined. A comprehensive multidisciplinary approach allows us to simultaneously address both needs.

Project Objectives

Objective 1: Develop soybean germplasm and varieties with reduced seed content of RFOs and increased sucrose content (>7%) in maturity groups 0-V, with acceptable oil and protein composition.

Objective 2: Quantify the metabolizable energy and the component pricing value of modified carbohydrate soybean meal.

Objective 3: Support Enhanced Nutritional Energy Meal (ENEM) market development with research data.

Project Deliverables

Objective 1 deliverables

- Licensing the released high sucrose and low stachyose variety, UA 5515HS in 2016.
- Re-evaluate and release a second high sucrose and low stachyose line, R07-2001, by summer 2017.
- Genetic mapping to identify and confirm genes for sucrose, stachyose, and protein and study the interaction among these traits by fall 2017.
- Enter advanced lines into the USDA Soybean Uniform test in 2018.
- Incorporate Rs2 alleles into southern-adapted germplasm by 2019.
- Soybean germplasm and advanced breeding lines with high sucrose (>7%) and low RFOs will be evaluated annually and released based on the best agronomic traits and carbohydrate profiles.
- Advanced lines entered into the USDA Soybean Uniform test in 2018, and annually thereafter.
- Germplasm for selection of breeding lines with modified soluble carbohydrates derived from diverse sources and using more than one mutation developed.
- Germplasm for selection of breeding lines with modified soluble carbohydrates plus higher protein developed.
- New lines with improved sugar, low oligosaccharide from Shannon’s breeding program will be evaluated annually and those lines with best agronomic traits and desired carbohydrate profiles will be released as varieties or germplasm.
- Enter advanced lines into the USDA Soybean Uniform test in 2018.
- Upon breeders’ sample nomination following the harvest season, MAS will be accordingly performed and reported to the breeders timely late 2016 and early 2017.
- From 100-150 selected progenies/breeding lines will be genotyped for each project cooperator.
- Each quarter of the FY 2017, 1,200-1,500 seed samples will be accurately phenotyped for carbohydrate composition and timely reported to the project PI as well as cooperators.
- Enhancement of NIR calibration for sucrose content through collaboration with breeders and Lorenz lab.
- December 2016 – 2017 Analyze sequence data and design new genotyping markers.
- March 2017 – Genotype pools and individuals for mutations.
- June 2017 – New mutants ready to plant for crossing in 2017 field season.
- Select and advance additional NILs with the variant allele combinations of the RS2 (W331-) and RS3 (snp6) genes that will target 200 pounds of each line to be available in early 2018 for Dr. Brake.
-January 2017-provide 100 pounds of down-regulated rs2 and control NIL line to Dr. Brake
-Select and advance additional NILs with the variant allele combinations of the RS2 (W331-) and -RS3 (snp6) genes that will target 200 pounds of each line to be available in January 2018 for Dr. Brake.
-January 2018-identification of >8% seed sucrose level from mutant lines.
- The advanced breeding populations and breeding lines will continue to be progressed through the breeding program until a variety release decision is made.
- Enter advanced lines into the USDA Soybean Uniform test in 2018.
- Move Rs2 into MG 0-1 germplasm by 2019.
Objective 2 deliverables

For all lines submitted for feeding studies and on an annual basis, a ME value index will be developed for each SBM relative to a control. This ME index will encompass both animal performance and digestibility data. In addition, for each line in each feeding study, kcal/lb. will be quantified that will serve as a target for improved soybean meal. Continue evaluation of economic value of identified ME increment relative to specific cultivars. Provide feedback to breeders.

- Annually, promote the most valuable lines in the project for quantification of metabolizable energy and the component pricing value of modified carbohydrate soybean meal.
- Produce seed for feeding studies to attempt to quantify the energy contribution of protein by conducting feeding studies with lines that have higher protein and typical soluble carbohydrate profiles.
Brake and Rainey:
By 2019, determine if modified soluble carbohydrate meal can provide 100 kcal/lb. of energy above commodity.

Chen, Lorenz, Rainey, Shannon:
The best lines developed by breeders will be increased and promoted for pilot projects based on performance data from USDA Uniform Tests.

Objective 3 deliverables

- By 2019, translate trait interactions and measures of stability in the nutritional bundle into practical economic targets for soluble carbohydrates soybean breeders.
- By 2019, make recommendations about the contribution of high sucrose to meal value.
- By 2019, interpret feeding study results in terms of achievable seed composition and quality.
- In 2017, initiate populations that combine known high sucrose QTL.

- Quantify value index of meal using data from with NILs, and data from lines with modified soluble carbohydrates, plus HOLL or higher protein.

- A subset of approx. 10 exotic germplasm with environmental protein and carbohydrate stability will be identified in late 2016.
- Several Dr. Shannon’s F5 progenies will be identified and confirmed for the germplasm and variety development in 2017.
- Several SNPs significantly associated with high sucrose QTL will be also identified and utilized for the marker development.
- Candidate causal genes of high sucrose can be detected in these QTL regions. The gene-based functional genetic markers will be developed for MAS.
- Two sucrose mapping populations (KB07-15 x PI 229343; SA12-1455 x PI 417015 and a diverse panel of 350 PI lines of the USB sequencing project will be phenotyped for genetic analysis and association mapping in 2017 by the Nguyen lab.
- For each mutant gene of rs2 and kti, NIL sets can be developed for each MG anticipated in summer 2017.
- Similarly, one or two NIL sets for high sucrose content can be developed based on the completion of QTL mapping anticipated in summer 2017.
- From the QTL mapping and GWAS performed in early 2017, either known QTL conferring high sucrose will be confirmed or novel QTL can be detected and genetically mapped in the soybean chromosomes.

- December 2016 – Plant some F1s (fad2-1a/fad21-b x rs2 W331 mutant, fad2-1a/fad21-b x rs3 G75 mutant) in greenhouse to advance to F2 generation.
- June 2017 – Plant remaining F1s (fad2-1a/fad21-b x rs2 W331 mutant, fad2-1a/fad21-b x rs3 G75 mutant) in the field, plant and genotype F2 from the greenhouse, and choose the appropriate individuals to cross with F1s (or parents) to generate triple mutants.
- Supply MG II germplasm lines to Dr. Rainey for final selection in 2018.
- MG III/IV lines will have 200 pounds of each NIL available in early 2019 for Dr. Brake.
Rainey, Bilyeu, Lorenz, and Chen:

- Two generations annually of NIL development in a range of maturity groups: Lorenz 0, I; Rainey II, III; Bilyeu III, IV; Chen IV, V.
- MG 0-V BC2S3 lines will be increased in 2019 for future studies.
Rainey and Lorenz:
- Develop a data set for prediction and association mapping of soluble carbohydrates in the USDA germplasm collection by 2018.
- By 2019, evaluate trait interactions (protein, seed size, etc.) with soluble carbohydrates in publicly available data sets and germplasm resources- SoyNAM, USDA germplasm collection, and/or USB core germplasm resequencing project.
- An association panel will be grown in 2017 and 2018. Results from this study will help understand the genetic architecture on the traits described.

Progress of Work

Updated September 11, 2019:

Updated September 11, 2019:

Final Project Results

Updated December 4, 2018:
See Year 3 Project for Final Report (FY19)

See Year 3 Project for Final Results (FY19)

Benefit to Soybean Farmers

In time, soybean varieties with more digestible sugars will be available to growers. This soybean will produce a superior meal that will be in demand by animal producers for inclusion in poultry and swine feed.

Performance Metrics

1. Working with USB staff we plan to share key results with a panel of soybean breeders and/or seed companies to measure the extent to which advancements in varieties and new sources of germplasm will be used toward the ultimate goal of advancing soybean value for farmers. Our goal is to identify at least 10 breeders that agree to use the materials developed through this project to advance toward commercialization of improved seed varieties.

2. With the help of USB staff, seek feedback from animal nutritionists, perhaps represented by the Animal Nutrition Working Group, that the advanced sucrose, lower raffinose/stachyose lines developed will provide added value to the animal feed industry.

Project Years