Project Details:

Identification of the chemical components of soybean seed and sprout related to anti-inflammatory activity

Parent Project: This is the first year of this project.
Checkoff Organization:Nebraska Soybean Board
Categories:Foods use
Organization Project Code:1738
Project Year:2019
Lead Principal Investigator:Toshihiro Obata (University of Nebraska)
Co-Principal Investigators:

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

The overall goal of the research team is to generate soybean germplasms for new food products with superior health benefits. To identify the promising target genes to be manipulated, metabolite and protein compositions of the soybean seed and sprout will be characterized and their relationship with dietary anti-inflammatory activity will be examined in the proposed project.

Project Objectives

The overall goal of the project is to improve the health beneficial effect of soybean derived bioactive food products in reducing the prevalence of chronic metabolic diseases, which is one of the major threats to the health of the US citizen. To this end, the objective of this proposed project is to identify the molecules in soybean and its sprout which show anti-inflammatory activities in human gut cells. We will further identify the genes responsible to the accumulation of these molecules, which will be the targets of genetic-based improvement of soybean seed composition for superior health benefits in the forthcoming projects.

Objective 1: Delineate the changes in chemical composition of soybean during sprouting and gastrointestinal digestion.
Objective 2: Evaluate the anti-inflammatory effect of the germinated whole beans in intestinal epithelial cells after simulated gastrointestinal digestion.
Objective 3: Identification of the chemical component responsible for anti-inflammatory activity and the genes regulating its accumulation.

Project Deliverables

Soybean sample preparation will be conducted within the first month of the project using the commercially available seeds. The preliminary experiments on the anti-inflammatory activity and composition of major chemicals will be performed within the first quarter of the project period. If promising candidate chemical is identified at this point, we will apply for the second year project to assess chemical diversity of seeds aiming to improve the health benefits of soybean. The rest of chemical and inflammatory parameters will continue through the second quarter of the project. The later half of the project duration will be used for the data analysis to identify the candidate chemicals and tests for their activities for the Objective 3.

Progress of Work

Final Project Results

Updated January 15, 2020:

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Benefit to Soybean Farmers

The experimental evidence on the health benefits of soybean and its sprout on the chronic metabolic diseases: Health benefits is an emerging issue when consumer choses dietary products. The scientifically demonstrated health benefit potentially impacts the consumer behavior to increase the consumption of soybean derived diets and market values of them. It should be noted that nearly 60 million individuals in the US are suffering from chronic metabolic disorders and the economic impact can be significant.

Promising target genes for the genetic-based improvement of health benefits of soybean: We will identify the genes regulating the accumulation of beneficial compounds in soybean. These genes are potential targets for breeding and/or transgenic generation of soybean cultivars with superior health benefits.

Performance Metrics

Project Years