Project Details:

Title:
Screening soybean germplasm and breeding soybeans for flood tolerance

Parent Project: This is the first year of this project.
Checkoff Organization:Mid-South Soybean Board
Categories:Water quality & management, Breeding & genetics, Environmental stress
Organization Project Code:MSSB
Project Year:2019
Lead Principal Investigator:Pengyin Chen (University of Missouri)
Co-Principal Investigators:
Keywords: water logging, flooding

Information and Results

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Project Summary

Waterlogging/flooding can be caused by poor drainage or excessive irrigation. Yield losses up to 50% are common in heavy, clay soils with poor surface and internal drainage. Breeding for waterlogging is a relatively new goal, and important in 2019 as farmers battle excessive rainfall. The highest yielding cultivar may not be the best choice on a poorly drained soil. We will screen current cultivars for tolerance and build breeding tools and germplasm for release to public and private institutions

Project Objectives

1) Screen current cultivars for flood tolerance
2) Differentiate tolerant and sensitive cultivars and PIs from the germplasm collection -- incorporate these genes into elite backgrounds
3) Recommend cultivars to mid-south farmers dealing with flood

Project Deliverables

We will develop flood tolerant germplasm and cultivars to be released and made available for crossing by private and public breeders. We will develop markers and tools for selecting superior lines for flood tolerance. We will also provide the farmers with rankings for currently available cultivars that are more tolerant to flooding.

Progress of Work

Updated February 6, 2020:
Quarterly Report April 2018

1. Evaluation of breeding lines for flooding tolerance and yield

i) Preliminary yield trial: A set of 11 breeding lines along with 5 commercial & susceptible checks will be evaluated for yield and flooding tolerance in clay soil during 2018 season. These 11 lines were selected from 2017 progeny rows from one flood tolerance population (S13-15826 x S13-15826). S13-15826 has flooding tolerance from R91-210-350 in its pedigree. The selection of these lines was done based on the flooding score of 1 to 2.5. The trial will be conducted in flooded field only.

ii) Advanced yield trial: Two advanced yield trails – MG-4 and MG-5 will be conducted in flooded field with clay soil and in two non-flooded fields having loam and clay soils. In MG-4 trial, 11 advanced breeding lines, and 3 commercial and one susceptible checks are included. Out of 11 lines, one line was derived from BC2 of S99-2281 × PI408105A, and the 10 high yielding lines that are included in 2018 Uniform Tests. In trial MG-5, 9 advanced breeding lines, and 2 commercial and one susceptible checks are included. Out of 9 advanced breeding lines, 4 lines were selected from 2017 AYT and the rest 5 are high-yielding lines that are included 2018 Uniform Tests. Both the advanced yield trials will be planted in 12’ 4-row plots with 3 replications. Data will be recorded on yield, maturity, plant height and lodging.

2. Flood yield evaluation trial for selected lines: This study includes18 breeding lines and 2 commercial checks. One half of the breeding lines is known to be flood tolerant while the other half is known to be flood susceptible. The test entries will be grown in three replications in flooded and non-flooded conditions in clay soil. The trial will be exposed to flooding stress in flooded field and flooding tolerance score will be recorded. The yield will be recorded from both flooded and non-flooded field. This is a multi-locations trial, and the Delta Research Center is one of the 4 locations.

3. Commercial variety evaluation for flooding tolerance: About 100-120 varieties (actual number is yet be known) developed by the different seed companies will be grown in 7-ft single row plots in three replications in clay soil. The field will be subjected to flooding stress at around R2 stage. The flooding tolerance score will be recorded about 10 days after draining flood water.

4. Genotype × Environment study: A set of about 120 accessions (PIs, RILs, and advanced breeding lines) will be planted in 7-ft single row plots and be subjected to flooding stress around R2 stage this season. The flood tolerance score will be recorded about 10 days after draining flood water. This is a multi-locations trial and the Delta Research Center is one of the 4 locations. The objective of the study is to identify most stable flood tolerant germplasm across the environments.

5. Field phenotyping of mapping populations: Two mapping populations – S99-2281 × PI567651 ((191 RILs), and V71-370 × PI407162 (292 RILs) will be planted in single row plots and be subjected to flooding stress around R2 stage for a period of 6-9 days this season. Flooding tolerance scores will be recorded on a scale of 1 to 5 where 1=tolerant to flooding while 5=very susceptible to flooding stress. The main objective of these studies is to map new flooding tolerance genes.

6. Evaluation of exotic lines for flooding tolerance: A set of 382 diverse plant introductions (PIs) used for genome sequencing (MG 4-6) will be grown in single row plots and be evaluated for flooding tolerance. The objective is to identify new sources for flooding tolerance with a view to use as parents in future crosses. The flooding tolerance score will also be used in genome wide association analysis.

Another set of 81 PIs of Glycine soja will be planted in hill plots and be exposed to flooding stress this season for flooding tolerance evaluation. The most tolerant soja soybeans could be used as parents to transfer flood tolerance to the elite breeding lines or could be used to develop mapping populations.

7. Crosses under generation advance: Currently there are four crosses are under generation advance. The crosses were made with a view to develop new flooding tolerant high yielding varieties and to develop mapping populations in order to map new genes from tolerant PIs. The generations of the crosses are being advanced in Costa Rica (CR) and Puerto Rico (PR).

8. New crossing plan in 2018: About 10-15 new crosses will be made between new high yielding elite breeding lines and flood tolerant PIs and breeding lines.

Updated February 6, 2020:
Quarterly Report June - September 2019

1. Evaluation of breeding lines for flooding tolerance and yield to develop commercial varieties

i) Advanced yield trial: A total of 34 high-yielding breeding lines in two groups: MG-4 (17 lines) and MG-5 (17 lines), developed by the University of Missouri, were subjected to flooding stress for about 2 weeks during R1-R2 stages. The flooding injury rating of these lines has been recorded. The same sets of lines are also being grown in non-flooded field for evaluation of yield and phenotypic acceptability. The results of the flooding injury data along with yield performance will be presented in the next quarterly report.

ii) Preliminary yield trial: A total of 64 breeding lines selected from 2018 progeny rows, in two maturity groups (MG 4 and 5) each comprising of 32 breeding lines, were subjected to flooding stress. The flooding injury rating of these lines and 4 commercial varieties has been recorded. The same sets of lines are also being grown in non-flooded field for evaluation of yield and phenotypic acceptability. The results of the flooding injury data along with yield evaluation will be presented in the next quarterly report.

2. Flood yield trial for selected tolerant and sensitive lines: This study includes 20 breeding lines and 4 commercial checks. The test entries were subjected to flooding stress and the flooding injury rating has been recorded. Seven lines, namely S13-15999, S15-3772RY, S14-16331, S11-20124C, R04-342, R06-4433 and R11-6870 exhibited flooding tolerance score from 1.3 to 2.0 (on rating scale of 1-5) with average score of 1.6 while commercial checks showed average score of 2.5. The sensitive line S99-2281 had a score of 4.3. The same set is grown under normal irrigation condition for yield evaluation. The yield performance along with yield index to be calculated based the head-to-head comparison between flooded test and non-flooded test, will be presented in next quarterly report. These lines are also being grown in other locations in AR, MS, LA and TX. The objectives of the trial are to evaluate the flooding tolerance stability across environments and effects of flooding stress on yield, seed quality and seed composition. Overall data will be analyzed and results presented in next report.

3. Screening recently developed elite lines for flood tolerance: A set of 100 breeding lines recently developed at the University of Missouri-Delta Research Center and at the University of Arkansas and five commercial cultivars as checks, are grown for flood tolerance evaluation. These lines were subjected to flooding stress, and flooding injury rating was recorded. Nine breeding lines, namely S16-14379C, S16-7922, S16-15170C, S17-19874R, S16-15896C, S16-11222, R15-7016, R15-7025 and UA 5814HP exhibited flooding tolerance score from 1.0 to 1.7 with average score of 1.3 while the commercial checks showed average tolerance score of 2.6. These lines are also being grown in Arkansas, Mississippi, Louisiana and Texas. Overall data will be analyzed and results will be included in next report.

4. Commercial variety testing for flooding tolerance: A set of 180 commercial varieties developed by different seed companies were subjected to flooding stress to evaluate their flooding tolerance. The flood injury rating data analysis results will be presented in the next quarterly report.

5. Evaluation of new breeding lines in progeny rows: A total of 648 progeny lines (F4:5) from 6 breeding populations, S14-16235 (FT) × S13-3851, S14-16267 (FT) × S15-12003, S14-16267 (FT) × V12-0045 R2, S14-36391 × S11-25615 (FT0, S14-9017RR × S11-25615 (FT), and S13-1955 × S16-16842 (FT) grown in 7’ single row plots were subjected to flooding stress. Flood injury rating of all the lines have been recorded. A total of 74 lines exhibited flooding tolerance with an injury score of ‘1’ on a rating scale of 1 to 5 (1 = No visible sign of flood injury while 5 = All leaves are yellow and plants are about to die).

6. Breeding population under generation advance: Twelve crosses were made in 2018 to develop new flooding tolerant high yielding soybean varieties from tolerant PIs and elite breeding lines in MO. These populations are being advanced from F1 to F4 in Costa Rica (CR) and Puerto Rica (PR), and expected to return homo station as F4:5 lines in April/May 2020 for evaluation and selection.

7. New crosses made in 2019 season: A total of 10 new crosses have been made between flood tolerant breeding lines and elite high-yielding breeding lines. In addition, 4 crosses involving 3 G. soja (wild) PIs have been made. F1 seed will be harvested in October and then sent to winter nursery for generation advance.

Final Project Results

Benefit to Soybean Farmers

Farmers in flood prone areas will be able to use these lines for improving on-farm profit and soybean production. This data can be used by public and private breeding programs to make selections beneficial to the farmer.

Performance Metrics

Project Years