2019
Isolation of soybean resistance (tolerance) genes against plant parasitic nematodes
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Contributor/Checkoff:
Alabama Soybean Producers
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Sang Wook Park, Auburn University
Co-Principal Investigators:
Kathy Lawrence, Auburn University
Edward Sikora, Auburn University
+1 More
Project Code:
Contributing Organization (Checkoff):
Alabama Soybean Producers
$13,000
Institution Funded:
Auburn University
$13,000
Brief Project Summary:

This effort aims at isolating resistance (tolerance) genes associated with plant roots to enhance resistance or tolerance of soybeans against nematodes, including soybean cyst nematode and reniform nematode. Determining the roles induced and/or preformed immunities in plant root and nematode interactions leads to evaluation of interdependences between root morphologies and root defenses against nematodes. This supports a long-term goal to generate new GM soybean plants with enhanced nematode resistance or tolerance.

Key Benefactors:
farmers, agronomists, Extension agents, geneticists, soybean breeders

Information And Results
Final Project Results

Update:
Preliminary results:
1) Root cells of soybean did not develop HR (localized cell necrosis) at or near the site of RN infections. Note that a) a number of studies have speculated that soybean and plant roots activate HR upon PPN infections, which is considered as the most eminent and effective immune machinery of plants against pathogens, and b) our concomitant experiments with cotton roots further supported that HR is not a part of plant defense mechanisms against PPN.

In particular, HR was not detectable until RN grew up to reach the pregnant stage. Considering that pregnancy takes a few days from the infection (J2) stage, this result clearly concludes that soybean roots are incapable of activate HR against PPN.

2) Root growth and shapes (preformed immunity) are correlated with plant tolerance against PPN attacks. In the concurrent studies with cotton roots, we noticed that PPN tolerant germplasm (Barbaren- 713) produces a larger number of root hairs than susceptible germplasms (Lonren-1, and Sure- Grow-714), suggesting the potential importance or roles of root morphology in plants’ tolerance against PPN infections.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.