Project Details:

Phenotyping recombinant inbred soybean lines (RIL) to identify molecular markers associated with charcoal rot resistance.

Parent Project: This is the first year of this project.
Checkoff Organization:Tennessee Soybean Promotion Board
Categories:Breeding & genetics
Organization Project Code:19-148-R
Project Year:2019
Lead Principal Investigator:Alemu Mengistu (USDA/ARS-West Tennessee Experiment Station)
Co-Principal Investigators:

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

The development of genetic resistance to major diseases of soybean is an essential component in soybean production and diseases management to reduce the potential impact on yield. Charcoal rot is a top five yield-reducer in the southern soybean production system. In 2015, nationally, charcoal rot resulted in approximately 11 million bushels lost. As a result of the continued yield losses due to charcoal rot, this proposal will provide new tools to reduce charcoal rot’s impact on soybean farmers.

Charcoal rot phyenotyping for the RILs were made at the West Tennessee Research and Education Center at Jackson. The soybean lines were grown and screened in charcoal rot infested filed. The stems were split and severity ratings were done. A portion of the lower stems and roots is being ground and plated using a selective media to determine the number of colony forming units of the fungus. The severity rating and the amount of colony forming units/gram of tissue will be used to determine whether a soybean line is resistant, moderately resistant, moderately susceptible or susceptible. The susceptible parents (Pharoah and Saline) used in these crosses have been shown in previous studies to be among the most susceptible to charcoal rot. The RILs will be genotyped at the USDA-ARS, Stoneville, MS using marker-trait association analysis using a genotype-by-sequencing to fine map resistance loci and develop breeder friendly markers.

The overall goal of this research is to provide producers with technologies to minimize soybean yield loss to the major soybean disease causing economic damage to Tennessee and the Midsouthern US.

Project Objectives

Objective 1: Phenotype recombinant inbred lines (RILs) to identify molecular markers that can be effectively used to incorporate charcoal rot resistance into breeding lines and to release resistant germplasm.

Objective 2: Determine the genetic inheritance for charcoal rot resistance.

Project Deliverables

Identify the genetics of resistance to charcoal rot and provide new markers for breeders to speed up variety release with pyramided resistance.

Progress of Work

Final Project Results

Benefit to Soybean Farmers

Performance Metrics

Project Years