Project Details:

Application of next-generation sequencing technology for accelerating soybean breeding for high yield and disease resistance

Parent Project: This is the first year of this project.
Checkoff Organization:Tennessee Soybean Promotion Board
Categories:Breeding & genetics
Organization Project Code:19-115-R
Project Year:2019
Lead Principal Investigator:Tarek Hewezi (University of Tennessee-Institute of Agriculture)
Co-Principal Investigators:

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

This renewal proposal uses a combination of plant breeding, molecular biology, bioinformatics, and genomic methodologies to discover new genes in order to improve soybean nodulation under a wide range of normal and stress conditions, Identifying genes that can be used to enhance nodulation efficiency and nitrogen fixation will certainly enhance amino acid biosynthesis and protein content in soybean seeds. During the last two years of this TSPB funding project we generated various libraries and completed the bioinformatics analysis of gene expression patterns, and epigenetic profiles of three nodule developmental states (initiation, formation, and senescence) for the first time in soybean. Also, we identified the complete set of differentially spliced genes (genes that produce different proteins) using a comprehensive platform of genomic and bioinformatics tools. These unique dataset place our team in excellent position to carry-out a functional characterization of gene candidates to prove their functions in enhancing nodule number, size, life span, nitrogen fixation and most importantly seed yield and quality as a logic next step of this project.

Project Objectives

Objective #1: Localize the expression of core and stage specific nodule genes using promotor::GUS assay.

Objective #2: Determine the impact of key nodule genes on nodulation efficiency and nitrogen fixation using overexpression and RNAi approaches.

Objective #3: Identify key regulatory genes and transcription factors that enhance nodule formation and development under stress conditions.

Project Deliverables

Progress of Work

Final Project Results

Benefit to Soybean Farmers

Performance Metrics

Project Years