Project Details:

Title:
An integrated approach to enhance durability of SCN resistance for long-term, strategic SCN management (Phase II)

Parent Project: An integrated approach to enhance durability of SCN resistance for long-term strategic SCN management (Phase II)
Checkoff Organization:North Central Soybean Research Program
Categories:Soybean diseases, Nematodes, Breeding & genetics
Organization Project Code:00067261
Project Year:2020
Lead Principal Investigator:Andrew Scaboo (University of Missouri)
Co-Principal Investigators:
Thomas Baum (Iowa State University)
Andrew Severin (Iowa State University)
Gregory Tylka (Iowa State University)
Melissa Mitchum (University of Georgia)
Brian Diers (University of Illinois at Urbana-Champaign)
Matthew Hudson (University of Illinois at Urbana-Champaign)
Show more
Keywords:

Contributing Organizations

Funding Institutions

Information and Results

Click a section heading to display its contents.

Project Summary

The soybean cyst nematode (SCN), or Heterodera glycines, is the most damaging pathogen to soybean production in North America. Current annual yield losses are estimated at more than $1.2 billion. Though SCN-resistant soybean varieties are available to minimize yield loss, producers are faced with limited options for rotation once virulent SCN populations develop in their fields. The widespread lack of genetic diversity for SCN resistance genes in commercial soybean varieties has significantly increased the prevalence of virulent SCN populations and reduced the effectiveness of current sources of resistance.

Thus, we have two major research challenges that, when successfully achieved, will enable us to develop more efficient management practices for this pest in the future.
1. Plant breeders need to increase the genetic diversity of SCN resistance in commercially available soybean varieties and work with nematologists to determine the most effective rotation practices that preserve the efficacy of the known sources of SCN resistance.
2. Nematologists need to identify the SCN genes required for the adaptation to reproduce on resistant varieties, use these as markers to monitor nematode population shifts in the field, and exploit this knowledge to help plant breeders identify the best resistance gene combinations for long-term nematode management.

To address these issues we are proposing Phase II of an integrated, collaborative, and multi-state project among plant breeders, molecular biologists, bioinformaticians, and nematologists. Our proposed objectives specifically address performance measures under Goals 1 (1.1, 1.2, 1.3), 3 (3.1, 3.2), 4 (4.6), and 5 (5.1) of the 2015-2020 SCN-Soybean North Central Research Program Strategic Plan and complement funding from federal agencies and the United Soybean Board. The genetic resources developed and knowledge gained from this project will provide immediate benefit to soybean producers and researchers in both the private and public sector.

Project Objectives

Objective 1. Diversify the genetic base of SCN resistance in soybean
• 1.1: Develop and evaluate germplasm with new combinations of resistance genes in high yielding backgrounds (Diers, Scaboo).

Objective 2. Identify SCN virulence genes to better understand how the nematode adapts to reproduce on resistant varieties
• 2.1: Sequence, curate and annotate SCN reference genomes for each common HG type (Severin, Hudson, Baum).
• 2.2: Generate sufficient genetic material of virulent SCN populations selected on different types of resistance (Mitchum, Baum).
• 2.3: Re-sequence the genomes and transcriptomes of virulent SCN populations and conduct comparative analyses (Severin, Hudson, Mitchum, Baum).
• 2.4: Validate and characterize genes associated with SCN virulence and evaluate their utility as novel resistance targets (Mitchum, Baum).

Objective 3. Determine what combinations of resistance genes would be beneficial in variety rotations to enhance the durability of SCN resistance in soybean.
• 3.1: Evaluate how rotations of various resistance gene combinations impact SCN field population densities and virulence profiles (Diers, Scaboo, Tylka, Mitchum).

Objective 4. Translate the results of objectives 1-3 to the SCN Coalition to increase the profitability of soybean for producers
• 4.1: Inform growers on effective rotation schemes designed to protect our current sources of resistance (Tylka, Mitchum).

Objective 5. Coordinate the testing of publicly developed SCN resistant experimental lines
• 5.1: Organize tests of experimental lines developed by public breeders in the north central US states and Ontario.

Project Deliverables

YEAR 1
Objective 1:
1.1 A population segregating for Rhg1 and Rhg4 from PI 437654, the genes on chromosomes 15 and 18 from G. soja, and a gene on chromosome 10 from PI 567516C will be yield evaluated in two locations to test for the impact of these resistance genes on yield. Breeding will be done to incorporate new combinations of resistance genes into high yielding genetic backgrounds.

Objective 2:
2.1 Create and release to collaborators draft genomes of at least five nematode HG types. Further develop SCN-Base cyber-infrastructure, GBrowse, tracks, using existing draft assembly and available SNP data/transcript data.
2.2 Further inbreed nematodes on soybean with different resistance genes and generate sufficient material for sequencing.
2.3 Extract DNA and RNA, begin genome re-sequencing and transcriptome analysis of virulent SCN populations selected on different resistance genes.

Objective 3:
3.1 Year 1 field trials to evaluate different rotation schemes for effectiveness at reducing population densities and the selection pressure on the nematode population.

Objective 5:
5.1 Tests of SCN resistant experimental lines will be coordinated.

YEAR 2
Objective 1:
1.1 A population segregating for Rhg1 and Rhg4 from PI 437654, the genes on chromosomes 15 and 18 from G. soja, and a gene on chromosome 10 from PI 567516C will be yield evaluated in two locations to test for the impact of these resistance genes on yield. Breeding will be done to incorporate new combinations of resistance genes into high yielding genetic backgrounds.

Objective 2:
2.3 Finish genome re-sequencing and transcriptome analysis of virulent SCN populations selected on different resistance genes and initiate comparative analyses. Deposit data to the SCN-Base website, and use to mine for candidate genes involved in SCN virulence.
2.4 Molecular, biochemical and/or genetic studies initiated on genes identified as putative virulence genes.

Objective 3:
3.1 Year 2 field trials to evaluate different rotation schemes for effectiveness at reducing population densities and the selection pressure on the nematode population.

Objective 5:
5.1 Tests of SCN resistant experimental lines will be coordinated.

YEAR 3
Objective 1:
1.1 Breeding will be done to incorporate new combinations of resistance genes into high yielding genetic backgrounds.

Objective 2:
2.1 Complete all sequencing and genome assemblies. SCN-Base will be updated with the final assembly and annotation of all genome sequences.
2.3. Continue comparative analysis using genomic sequences to identify genes under selection.
2.4 Genes identified as putative virulence genes will be further characterized using molecular, biochemical and/or genetic analysis.

Objective 3:
3.1 Year 3 field trials to evaluate different rotation schemes for effectiveness at reducing population densities and the selection pressure on the nematode population.

Objective 4:
4.1 Results communicated to SCN Coalition

Objective 5:
5.1 Tests of SCN resistant experimental lines will be coordinated.

Progress of Work

Updated July 17, 2020:
Objective 1: Diversify the genetic base of SCN resistance in soybean
1.1: Develop and evaluate germplasm with new combinations of resistance genes in high-yielding backgrounds

Diers – Our research is continuing to develop new varieties with non-PI88788 sources of resistance. New, high yielding lines with SCN resistance from non-PI 88788 sources were selected in 2019. Five new high yielding lines that have Rhg1-b from PI 88788 combined with two resistance genes from G. soja were selected for testing in uniform or regional test this summer.

Scaboo – We are developing germplasm and varieties in the relative maturities 3.5 to 4.2 with resistance to SCN HG 1.2.5.7 (Race 2), which is currently the predominate HG type in Missouri. We are using novel SCN resistant sources including PI 437654, PI 90763 and PI 468916, with most support from USB and MSMC for these efforts. We will continue population development by forward and backcrossing, using phenotypic screening and molecular markers for SCN resistance selection, and by yield testing elite germplasm in both state and regional tests (run by Diers). Our first yield tests of novel soybean material with Race 2 resistance was during the summer of 2019, and we plan on having this material in various stages of yield testing every year thereafter.

Objective 2: Identify SCN virulence genes to better understand how the nematode adapts to reproduce on resistant varieties.
2.1: Sequence, curate and annotate SCN reference genomes for each common HG type

Baum/Severin – One of the major milestones in SCN biology is developing a completely annotated SCN reference genome, which we have recently accomplished (Masonbrink et al., 2019). Using NCSRP funds we continue to refine this resource further. We have made considerable progress in that direction and now have a chromosome level genome assembly ready and annotated. Currently, we are preparing a manuscript describing these results to be submitted shortly. Simultaneously, we have developed a centralized, web-based repository called SCNBase (SCNBase.org). We have developed this web portal de novo and published it in November 2019 (https://doi.org/10.1093/database/baz111). This web portal now is the home to all bioinformatic, genetic, genomic, and molecular data generated for SCN, most of it through soybean check-off funding. Using this web portal, researchers and breeders from all over the world can access and analyze all public bioinformatic data generated in this proposal and curated from previous research at the nucleotide level. We are already seeing significant “web traffic” to this web portal suggesting that it is generating considerable interest in the SCN community from all over the world. For example, since SCNBase has been open to the public earlier in 2019 it has been accessed by 1,792 unique users. During the period of January 1 to March 26, 2020 alone, this web portal had 307 unique users, almost half of which were located outside of USA. As such, SCNBase provides prime visibility and impact of the collective data procured through farmer investments in research, which and will enable others in a coordinated fight against this pathogen. This current NCSRP project is already generating and will continue to generate thousands of gigabytes of data, which will be incorporated into SCNBase as we continue to develop this web portal.

Hudson – Our project has now generated high quality genome sequences for five SCN isolates: OP50, TN 7, 8, 20, and 22. These sequences are all more complete than the TN10 sequence published by our consortium last year. The TN10 reference has now been improved to comparable or better quality by the Iowa group. We thus have six complete genomes of different SCN strains with assemblies each with N50 > 10MB, plus the Chinese group’s assembly of the X1 strain, which is less reliable than those from our own project. The five completed sequences have been annotated and effector sequences have been identified using sequence similarity to a set of 80 curated plant parasitic nematode effectors. So far, the variability of size in the seven different assemblies, and the sequencing and assembly methods used, seem to be affecting the overall number of genes and effector genes more than the likely biological differences. We are in the process of curating the assemblies to make them more comparable. However, we are able to identify the presence of between 63 and 69 of the 80 effector families in each genome, giving each isolate a substantial arsenal of effector proteins. The correlation between effector families in the genome and virulence on specific hosts is an ongoing analysis expected to generate preliminary data by September 2020. We are in the process of repeating and enhancing these methods on two more isolates, MM26 and PA3. We are using the new PacBio sequencer which should produce even better data than the nanopore sequencing used previously. Sequencing is completed for PA3 and in progress for MM26. Assembly for PA3 has begun. Both genomes are expected to be completed by September 2020, giving us high quality whole-genome sequences of all the key HG types.

Mitchum – Continuously maintained live cultures, prepped and provided all of the genetic material for each common HG type in sufficient quantities for various sequencing platforms used to generate the reference genomes described above.

2.2: Generate sufficient genetic material of virulent SCN populations selected on different types of resistance

Mitchum – In Phase I of this project (under Obj 2.3 of the previous project) we identified a HG type 1.2.5.7 field population and continuously selected this population in the greenhouse on either a susceptible soybean line (SCN inbred population MM-BD1), a soybean line containing the Rhg1 resistance gene from PI 88788 (SCN inbred population MM-BD2), a soybean line containing the Rhg1 and Rhg4 resistance genes from PI 437654 (Hartwig) (SCN inbred population MM-BD3), and a soybean line containing resistance genes on chromosomes 15 and 18 from wild soybean G. soja (inbred population MM-BD4) for more than 12 generations. The original SCN field population and this series of SCN populations selected for virulence on each set of resistance genes has been continuously reared to generate sufficient genetic material for sequencing. The populations were subjected to another 6 months of selection in this project period, HG typed, and frozen.

2.3: Resequence the genomes and transcriptomes of virulent SCN populations described in 2.2 and conduct comparative analyses

Baum/Severin – Our group is actively involved in developing, analyzing and comparing gene expression in from virulent (i.e., able to infect resistant soybean cultivars) and avirulent (i.e., unable to infect resistant soybean cultivars) SCN populations with the goal to identify the genetic determinants of virulence. For that purpose, we are focusing on three individual gland cells, in which the nematode produces the effectors/tools required for infection and defense suppression, using a method we previously had developed. Toward that end, we have completed three independent biological replications of mRNA purification, library construction and sequencing (which is essentially an identification of all genes expressed in the gland cells at a given time) from each of the virulent (MM10) and avirulent (PA3) SCN populations. Furthermore, gene identities from all sequencing experiments have been generated utilizing the SCN TN10 genome produced by us (Masonbrink et al., 2019). We identified 13,617 unique transcripts from the PA3 population and 8,820 unique transcripts from the MM10 population, which represent a snapshot of gene activity during the early parasitic stage of SCN infection (parasitic J2). As a point of validation, we have identified all previously discovered SCN effectors expressed during this early parasitic stage within our gland cell-specific sequence data. Initial analysis of these sequence data has revealed intriguing gene expression differences between these virulent and avirulent SCN populations. Specifically, there are 32 genes upregulated in the SCN PA3 libraries versus the MM10 libraries. Protein products of 13 of these genes are predicted to be nuclear localized and likely have gene regulatory functions. This includes a diverse group of proteins involved in functions such as structural maintenance of chromosomes, splicing factors, a glycosyltransferase involved in increased virulence in other parasitic nematodes, DNA-binding proteins, and a ‘ran’-binding protein, among others. Conversely, we discovered 17 genes that are upregulated in SCN MM10 versus PA3. Protein products of eight of these genes are predicted to be nuclear localized. Among this group are previously reported effectors, a microtubule binding protein homolog from an animal-parasitic nematode, signal transduction proteins, and several unknown proteins. We are still at the initial stages of analysis but it is already clear that we have generated a rich source of critical data needed to understand SCN virulence.

Mitchum – The MM26 parental population for the adapted populations outlined in 2.2 above was prepared and sent for genome sequencing; the others will follow. RNA-seq data generated from early parasitic life stages of a virulent and avirulent population of SCN were used to conduct a reference-based transcriptomic analysis with the aid of the SCN genome. We identified 207 genes unique to the virulent SCN that could be turned on to overcome resistance mechanisms; conversely, 92 genes that could be turned off to evade triggering resistance mechanisms.
Hired a new postdoctoral research associate with expertise in advanced bioinformatics, population genetics, and genomics. He will focus on extending our work on the molecular genetic and comparative genomics analysis of soybean cyst nematode population structure as it relates to virulence on resistant host plants. Unfortunately, we are now faced with a significant delay in his joining our team due to COVID-19.
We continued our collaborative effort with the Baum lab to generate nematode gland-specific RNA-seq datasets of a virulent and avirulent population of SCN; several joint meetings were held to discuss comparative analyses which are underway to identify differentially expressed gene candidates with a potential role in virulence using the newly annotated pseudomolecule SCN genome assembly.

2.4: Validate and characterize genes associated with SCN virulence and evaluate their utility as novel resistance targets

Mitchum – As candidate SCN virulence genes are identified, gene function will be confirmed through biochemical and/or genetic analysis to not only better understand the mechanisms of virulence, but to also evaluate these gene targets as vulnerable points of disruption in the SCN life cycle as a means to enhance resistance in soybean. We focused our efforts on validating the genes identified in 2.3 above to prioritize candidate virulence genes for further molecular functional studies.

Baum – Being a sedentary endoparasite that relies exclusively on its host for survival, SCN has to suppress host defense responses for a significant duration in order to survive. The SCN achieves this by producing a large number of effector molecules and delivering them into the soybean cells via its mouth spear. These effectors specifically target host factors and modulate their functions, thereby also altering soybean defense responses. Generating an in-depth understanding of how individual effectors help SCN establish and maintain infection is a very difficult but necessary task that will reveal vulnerable “nodes” in host defense pathways that can be strengthened via either breeding or molecular approaches.
As a part of this project, we are actively involved in conducting in-depth molecular characterization of SCN effectors specifically involved in host defense suppression. For this reporting period, we specifically focused on the effector named 28B03. We have identified that this particular effector is a robust host defense suppressor. We have observed that due to its function, plants become more susceptible to cyst nematodes. We have also identified that this effector specifically targets a previously uncharacterized plant protein kinase. By physically interacting with this novel protein kinase, the 28B03 effector suppresses phosphorylation of its substrate protein and completely alters the associated signal transduction pathway. This discovery and an in-depth molecular characterization of a novel defense response related kinase cascade in plants are breakthrough discoveries of this study. We are currently writing a manuscript describing these results. Simultaneously, we are gearing up to conduct another high-impact protein interaction experiment in order to identify the complete ‘interactome’ of the proteins associated with this kinase cascade.

Objective 3. Determine what combinations of resistance genes would be beneficial in variety rotations to enhance the durability of SCN resistance in soybean.
3.1 Evaluate how rotations of various resistance gene combinations impact SCN field population densities and virulence profiles

Diers – The Illinois location of the rotation trial was harvested and fall soil samples were taken to evaluate SCN population changes over the summer. Samples from susceptible checks show that the SCN population was an HG type 2 (Race 5) in two plots and a HG type 1.2 (Race 2) in one plot. The analysis of the change in egg number between the spring to the fall show that there was a 5 X increase in eggs through the growing season for the susceptible check, a 3.8 X increase for lines with only Rhg1b, a 1.6 X increase for lines with Rhg1b plus two QTL from G. soja, a 3 X increase for Rhg1b plus two QTL from G. soja and a fourth QTL on chromosome 10, a 2 X increase for lines with Rhg1a + Rhg4 from Peking, and finally only a 0.3 X change for PI 90763. These results are close to what we expected and show that the different sources influenced SCN reproduction in the field. Preparations are being made to plant the second year of the test in the field.

Scaboo/Mitchum – Microplot field experiments were established to evaluate how rotations of SCN resistance gene combinations impact SCN field population densities and virulence profiles. Treatments with resistance genes: Rhg1-a, Rhg1-b, Rhg4, cqSCN-006/cqSCN-007, and the chromosome 10 QTL, along with susceptible and resistant checks were designed to form 12 treatments with 3 replications in a randomized complete block design. Hand planting and inoculation were done on May 31st, 2019. Soil samples from all 36 plots were obtained directly after inoculation to determine the baseline of SCN egg density. A moderate level of infestation was achieved. At 60 days post-inoculation (DPI), soil samples from the three susceptible plots and one field sample collected between the microplots were collected to determine the initial HG type. SCN HG type at 60 DPI was the same for all three susceptible plots. The HG type for these plots was HG type 2.5.7, which was similar to the SCN population present in the field location between plots. At the end of the growing season, soil samples from all plots were obtained to determine egg density and SCN HG type at harvest. All resistance genotypes had a reduction in egg densities and the susceptible treatment had an significant increase in SCN egg density, while PI 90763 had the greatest percentage of egg count reduction followed by genotypes with resistance genes, Rhg1a + Rhg4. SCN HG type results at the end of the first growing season show no specific trend.

Tylka – At the conclusion of the 2019 growing season, two separate soil samples were taken from each microplot in both experiments conducted in Iowa. The first set of soil samples were used to obtain an end-of-season population density of SCN in each plot, and the second set of samples were sent to the University of Missouri for HG Type testing to determine if and how the soybean genotypes grown in the microplots in 2019 might have shifted the virulence phenotypes (HG types) of the SCN populations added to the microplots in the spring of 2019. Preliminary data analysis was completed for each of the experiments in Iowa, and some trends in SCN population density were observed. In both experiments, the highest SCN population densities were found in microplots in which the susceptible soybean variety was grown. The second highest SCN population densities were found in microplots in which soybeans with only Rhg1b resistance were grown. The lowest population densities were found in plots where PI 90763 and Rhg1b+soja+ch10 resistance were grown. The results of the HG Type test results on the SCN populations in the soil samples collected from the microplots at harvest in 2019 are not yet available. Preparation and planning for the second growing season has begun with the arrival of seed.

Objective 4. Translate the results of Objectives 1-3 to the SCN Coalition to increase the profitability of soybean for producers.
4.1: Inform growers on effective rotation schemes designed to protect our resistant sources

Tylka/Mitchum – A news release about stacking SCN genetic resistance and rotation strategies was created and disseminated in 2019 and it led to interviews with Melissa Mitchum on Brownfield Ag Network and Brian Diers on Adams on Agriculture radio programs, and an interview by Melissa Mitchum with Successful Farming/Agriculture.com (print/digital media). Also, the February 2020 issue of Progressive Farmer magazine had a special section titled “Crop Invaders”, and it contained four pages devoted to SCN resistance (see Appendix) and an article titled “A New Movement: The Push for SCN Varietal Resistance Broadens” in which Brian Diers discusses novel SCN resistance genes and rotating their use to preserve their effectiveness. Also, our NCSRP-funded research was mentioned in radio and print interviews with Greg Tylka by the following media at the Commodity Classic meeting in San Antonio, February 27-28, 2020: Emily Unglesbee, DTN; Gil Gullickson, Successful Farming; Mike Perrine, MP Ag Radio; Michelle Rook, WNAX; Mike Adams, Adams on Ag; Anna Hastert, Iowa Agribusiness Radio; DeLoss Janke, Illinois FB radio; Clinton Griffiths, Farm Journal; George Bower, KICD radio, Spencer; Ashley Davenport, Michigan Ag Today radio; and Mick Kjar, AgNews 890.

Objective 5. Coordinate the testing of publicly developed SCN resistant experimental lines.
5.1: Organize tests of experimental lines developed by public breeders in the north central US states and Ontario

Diers – The report from the 2019 SCN Regional Test was completed and the first version of the results was sent to all test collaborators on 19 December 2019. This version included the agronomic, composition and resistance test results. This version was followed by the distribution of the final version on 6 January 2019 which included the results of testing the egg number and HG type of nematode populations in field environments. This quick reporting of the 2019 results is important for breeders so this information can be used in release and crossing decisions. Planning has taken place for the 2020 tests and entry lists for the trials have been made and seed has been distributed to cooperators. The 2020 tests will include 194 entries that range from maturity groups 00 to IV. The test will be grown in 32 environments in 10 states and one Canadian province. Because of the Covid 19 outbreak, it is not known how many locations will be planted.

Please see attachment for addition information and Appendix.

View uploaded report PDF file

Final Project Results

Benefit to Soybean Farmers

This project will benefit soybean producers by creating a long term management strategy for SCN through knowledge and soybean germplasm development.

Performance Metrics

Objective 1: Diversify the genetic base of SCN resistance in soybean.
Accomplishments: In Phase I of this project, we developed experimental lines with new combinations of resistance genes. Genes that were combined included Rhg1 from PI 88788 or PI 437654 (Hartwig), Rhg4 from PI 437654, genes on chromosomes 15 and 18 from wild soybean G. soja, a gene from PI 437654 on chromosome 11, and genes on chromosomes 10 and 18 from PI 567516C. Combinations of these genes were tested for SCN resistance. Associations with yield in SCN-infested environments were made to determine whether these resistance genes are associated with greater yield. Gene combinations effective in controlling SCN are being bred into elite soybean germplasm using marker-assisted selection.

Objective 2: Identify SCN virulence genes to better understand how the nematode adapts to reproduce on resistant varieties.
Accomplishments: In Phase I of the project we used innovative methods and new sequencing technologies to enhance and complete the current SCN reference genome assembly for the TN10 population (HG type 0) and the sequence of its bacterial endosymbiont. Additionally, we used state of the art bioinformatics tools to make substantial progress to annotate and curate the SCN genome. We generated a comprehensive SCN gene expression atlas from whole nematodes, which was also used in the annotation of the SCN genome. We created a genomic toolbox for SCN (SCN-Base) that facilitates the integration of very large sequence data sets, molecular markers, QTL data and genetic maps into an easy-to-use web interface. This resource will soon be released as a website that includes a Genome Browser for easy visualization of the SCN genome. We also developed lower-cost methods to produce reference genomes of additional HG types.

Objective 3. Determine what combinations of resistance genes would be beneficial in variety rotations to enhance the durability of SCN resistance in soybean.
Accomplishments: As described above, experimental lines with resistance gene combinations developed in Objective 1 during Phase I of this project were tested in four different rotation schemes with experimental lines containing various resistance gene combinations in a greenhouse study. SCN population increase was measured after each generation for 8 generations. Following the eighth generation of selection, the HG type of each population was determined. From this, we identified alternative resistance gene combinations that when used in rotation reduce the selection pressure on the SCN population thereby slowing nematode adaptation to resistant varieties.

Objective 4. Translate the results of objectives 1-3 to the SCN Coalition to increase the profitability of soybean for producers.
Accomplishments: In Phase I of the project, an extension and outreach coordinator, advised by project Co-PI Dr. Tylka, was hired to provide farmer education and outreach for the project. A survey of extension and outreach educational materials about SCN biology and management in the NCSRP states was conducted by the coordinator. Materials from land-grant universities and private seed and chemical companies were gathered, analyzed, and compared.

Objective 5. Coordinate the testing of publicly developed SCN resistant experimental lines.
Accomplishments: During 2018, the testing of SCN resistant experimental lines developed by breeders in 11 north central US states and Ontario was coordinated. The tests include 182 SCN resistant experimental lines and varieties that are being grown in 104 maturity group specific trials across 39 locations. These lines are also being tested for SCN resistance and the soil samples from the environments will be evaluated for SCN population density and HG type.

Project Years