Project Details:

HOLL plus soybean variety development

Parent Project: This is the first year of this project.
Checkoff Organization:Missouri Soybean Merchandising Council
Categories:Breeding & genetics, Seed composition
Organization Project Code:454-21
Project Year:2021
Lead Principal Investigator:Andrew Scaboo (University of Missouri)
Co-Principal Investigators:
Keywords: biotechnology

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

This proposal is a research and variety/germplasm development program to create competitive yielding
soybean lines with the high oleic/low linolenic (HOLL) trait plus additional seed composition traits that will
enhance the value of the soybean to the producer, processor, and end user. This project is part of a multi-year
effort to support the University of Missouri breeding program in developing varieties with added profit
potential for Missouri producers.

Project Objectives

The objective of this proposal is to establish a HOLL plus soybean breeding and molecular selection program dedicated to developing high yielding maturity group III and IV soybean varieties for Missouri farmers.

Project Deliverables

competitive yielding soybean lines

Progress of Work

Updated April 27, 2021:
This project is designed to investigate soybean quality traits that may be added to increase the value of the high oleic,
low linolenic (HOLL) trait.

We have completed the 2020 harvest, some of the seed composition analyses, and winter nursery seed generation for 2021 yield tests and progeny row evaluations for our HOLL Plus stacks corresponding to the five or six targeted genes. From the 571 plant rows with the four HOLL Plus combinations, we selected 174 for further analysis. We completed fatty acid analyses and NIR assessments of protein, oil, and other seed qualities.

The HOLL Plus SATS lines achieved both very high oleic acid and low linolenic acid levels and met the threshold of <7.3% saturated fatty acids that enables labeling the oil consistent with FDA rules for “low in saturated fats” and the qualified health claim for high oleic acid oils. The HOLL Plus LIPOX and HOLL Plus VITE lines were HOLL, but their Plus seed composition traits were not evaluated. We used the fatty acid analysis to select HOLL Plus VITE lines that contained all of the targeted genes for the HOLL trait from those that were noted to still be segregating for one gene. The CARB Plus lines had high oleic acid levels. We discovered a genotyping error during line selection that resulted in only one of the two genes for low linolenic acid being present in this germplasm, and the slightly elevated linolenic acid results reflect the impact of missing the second gene. Fatty acid analyses were conducted on all HOLL Plus lines targeted for progeny rows for 2021.

Final Project Results

Benefit to Soybean Farmers

Investing in continued value acquisition is critical to the long term success of soybean as a profitable crop.

Performance Metrics

Project Years