Updated December 7, 2021:
We generated a total of 11 novel genotypes of soybean having CRISPR-induced deletions in 10 genes. These genotypes have been phenotyped to access any effect of the mutations on plant growth and seed quality. Our phenotypic data showed no deleterious effect on plant growth and, more importantly, improved seed quality was observed. We also performed semi-quantitative seed proteomics on the mutant genotypes and identified the proteins whose production was decreased or increased in the mutants compared to wild-type controls. Moreover, we are in the process of generating additional transgenic plants to further enhance the improved seed quality we observed so far.