Climate change has threatened soybean production and affected soybean growers’ profitability in the U.S. Increasing genetic yield potential and improving climate resilience using diverse soybean germplasm are important goals in soybean breeding and critical for soybean production in the U.S. With funding support from the United Soybean Board over the past several years, the team has successfully developed a strong pipeline of elite soybean materials with high-yield, climate-resilience and diverse pedigrees and novel breeding technologies and tools to support breeding efforts. Based on previous discoveries and research foundation, seven scientists from six soybean-growing states across MG 00 to VIII will work as a team in this proposal to achieve the following objectives: 1) evaluate and release high yielding soybean varieties with regional adaptation and climate resilience across MG 00 to VIII; 2) develop soybean germplasm with diverse genetic background for climate resilience; and 3) discover novel genes associated with climate resilience using emerging technologies and methodologies to support the breeding efforts. The outcomes generated from this proposal include released varieties/germplasm with wide regional adaptation for commercial production and next-gen breeding materials, and novel genes associated with climate resilience. This work will benefit soybean growers in the U.S. by providing new soybean varieties adapted to regional growing conditions with tolerance to climate changes. The project will also provide new and improved germplasm to the commercial and public breeders for use as parental stocks. Novel genes associated with climate resilience will benefit soybean researchers, enabling accelerated genetic gains.