Project Details:

Characterization of the mechanism involved in the SDS-SCN interaction to develop soybean lines with resistance to SDS and to SCN

Parent Project: This is the first year of this project.
Checkoff Organization:Iowa Soybean Association
Categories:Soybean diseases, Nematodes
Organization Project Code:
Project Year:2013
Lead Principal Investigator:Leonor Leandro (Iowa State University)
Co-Principal Investigators:
Silvia Cianzio (Iowa State University)
Gregory Tylka (Iowa State University)
Osman Radwan (University of Illinois-Carbondale)
Keywords: Disease Interactions, Soybean Cyst Nematode (SCN), Sudden Death Syndrome (SDS), Sudden Death Syndrome - SCN Interaction

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

The main goal of this research is to reduce soybean yield losses due to SCN and SDS. This goal will be achieved by characterizing the response of RILs to infection by the two pathogens, alone and in combination. Molecular characterization of the mechanism of the interaction will also facilitate future breeding efforts to release soybean lines with resistance to both pathogens.

Project Objectives

1. Determine if the SCN-SDS interaction requires the physical presence of both pathogens on a root or is it mediated by a systemic host response.
2. Understand the molecular interactions between SCN and SDS by comparing expression in response to infection by both pathogens.

Project Deliverables

Progress of Work

Seed of four recombinant inbred lines (RIL’s) with different combinations of resistance to SDS and SCN were obtained by harvesting a new generation of plants grown at an ISU greenhouse. This was necessary because seed germination and vigor was very poor in the initial batch of seeds. A protocol was developed that facilitates simultaneous infection of the RILs by both pathogens, so that gene expression can be measured at early stages of soybean defense response to infection. The first replication of the experiment was established at the ISU Plant Pathology greenhouse but was unsuccessful due to sudden temperature drop that caused seedling hypocotyls to snap. New inoculum is being prepared to repeat the experiment.

Protocols for testing the interaction at the molecular level were discussed between the ISU and UIUC teams. It was decided that the inoculations for this purpose would be done in the lab in paper towels and that SCN juveniles would be used. This will allow us more control on the timing of infection and sampling roots at time periods when gene expression will be optimal.

Final Project Results

Benefit to Soybean Farmers

Performance Metrics

Project Years