Project Details:

Increasing profits through genetic resistance to SDS

Parent Project: This is the first year of this project.
Checkoff Organization:North Central Soybean Research Program
Categories:Breeding & genetics, Soybean diseases
Organization Project Code:
Project Year:2014
Lead Principal Investigator:Brian Diers (University of Illinois at Urbana-Champaign)
Co-Principal Investigators:
Dechun Wang (Michigan State University)
Jason Bond (Southern Illinois University at Carbondale)
Osman Radwan (University of Illinois-Carbondale)
Glen Hartman (USDA/ARS-University of Illinois)
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Keywords: Sudden Death Syndrome (SDS)

Contributing Organizations

Funding Institutions

Information and Results

Comprehensive project details are posted online for three-years only, and final reports indefinitely. For more information on this project please contact this state soybean organization.

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Final Project Results

Three mapping populations with a total of 559 lines developed using the two new resistance sources GD2422 and E07080 were evaluated for SDS resistance reactions in the MSU SDS disease nursery near Decatur, MI. Marker and SDS data from the two GD2422 populations were analyzed and 11 chromosomal regions were found to be associated with SDS resistance.

Previously mapped SDS resistance genes were retested for resistance reactions in populations developed so the genes could be confirmed in uniform genetic backgrounds. These tests confirmed the effect of two of the five genes tested. A third gene was found to be significant for resistance, but the effect of the gene was opposite of predictions, meaning that the resistance allele was associated with more disease than the allele for susceptibility. The confirmed genes will be useful to select in plant breeding programs.

Resistant and susceptible near-isogenic lines (NILs), parents of the NILs, and resistant and susceptible checks were tested in a greenhouse for SDS resistance to verify their resistance reactions. In a third assay, root and leaf tissue from infected and non-infected plants were collected at three time points and tested for gene expression to determine when tissue sampling for gene expression assays should be done. Results from these tests indicate that samples should be taken 6 to 10 days after inoculation with Fusarium virguliforme, the fungus that causes SDS.

Project Years