Project Details:

Genetics of Multi-Soybean Aphid Biotype Resistance and Soybean Aphid Virulence on Rag Genes (Year 1 of 1420-532-5605)

Parent Project: This is the first year of this project.
Checkoff Organization:United Soybean Board
Categories:Breeding & genetics, Insects and pests
Organization Project Code:1420-532-5605
Project Year:2014
Lead Principal Investigator:Darin Eastburn (University of Illinois-Carbondale)
Co-Principal Investigators:
Anitha Chirumamilla ((not specified))
Glen Hartman (USDA/ARS-University of Illinois)
Keywords: Soybean Aphid - Biotypes, Soybean Aphid - Genetic Resistance

Contributing Organizations

Funding Institutions

Information and Results

Comprehensive project details are posted online for three-years only, and final reports indefinitely. For more information on this project please contact this state soybean organization.

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Final Project Results

Achieving the objectives
- Six PI accessions found to be resistant to biotype 1, 2 and 3 aphids in earlier studies were shown to be resistant to biotype 4 as well, indicating the these PI lines are exhibiting different Rag alleles for Rag1 and Rag2 than earlier resistance sources.
- Clones from biotype 2 x 1, 2 x 3 and 3 x 2 crosses were established in field buckthorn cages.
- Three field-collected aphid isolates were tested and determined to colonize Williams 82, Rag1 and Rag2 lines, indicating that all three exhibited biotype 4 virulence.
- Will attempt to mate soybean aphid with nastursium and cotton-melon aphids since they all use the same buckthorn over-wintering host in an effort to see whether hybridization could be impacting virulence.
- Three lines with rag1c and rag4 resistance from PI 567541B were selected for advanced evaluation of agronomic performance in 2015.
- The rag1c and rag4 resistance genes from PI 567541B and Rag3 from PI 567543C were fine-mapped within genetic intervals less than 100 kb.
- An experimental protocol has been developed to isolate biochemicals associated with aphid virulence against specific soybean aphid resistance genes.

Project Years