Project Details:

Title:
Screening for genetic resistance against soybean viruses

Parent Project: Screening for genetic resistance against soybean viruses
Checkoff Organization:North Central Soybean Research Program
Categories:Breeding & genetics, Soybean diseases
Organization Project Code:
Project Year:2012
Lead Principal Investigator:John Hill (Iowa State University)
Co-Principal Investigators:
Steve Whitham (Iowa State University)
Reza Hajimorad (University of Tennessee-Institute of Agriculture)
Craig Grau (University of Wisconsin)
Keywords: Alfalfa Mosaic Virus (AMV), Bean Pod Mottle Virus (BPMV), Soybean Viruses

Contributing Organizations

Funding Institutions

Information and Results

Comprehensive project details are posted online for three-years only, and final reports indefinitely. For more information on this project please contact this state soybean organization.

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Final Project Results

Bean Pod Mottle Virus: (BPMV)
• Evaluated several hundred recombinant inbred lines for tolerance/resistance to BPMV using BPMV engineered to contain a gene herbicide resistance. Identified numerous (ca. 90) lines that showed resistance/tolerance to BPMV

• Selected a subset of lines using criteria of seed quality, yield loss, and symptom severity. Severity scale used is 0 (no symptoms) to 3 (severe symptoms). Average yield loss scale 1 – 31%, scale 2 = 39%, scale 3 = 45%. Some lines showed 0% yield loss.

• Some lines may exhibit complete resistance to BPMV

• Seed from lines are being assayed by quantitative ELISA technique developed to identify tolerance/resistance. Assays are complete. Data analysis is in progress

Alfalfa Mosaic Virus (AMV)
• Produced antiserum to AMV using a novel approach expressing part of AMV in a bacterium and using for antibody production. Antibodies are effective for detection of a large number of AMV strains.

• Identified sampling time for AMV detection relative to soybean leaf position and AMV inoculation time. Data suggest leaflet collections should be made pre-bloom.

• Seed-borne nature of AMV along with symptomless AMV isolates suggest conclusions based upon soybean symptom expression regarding susceptibility and potential for seed transmission may be erroneous.

Project Years