Project Details:

Development of high-yielding, high-protein germplasm by enhancing nitrogen acquisition and its transport to seed (Year 2 of 1820-152-0136)

Parent Project: Development of High-Yielding, High-Protein Germplasm by Enhancing N Acquisition and its Transport to Seed (Year 1 of 1820-152-0136)
Checkoff Organization:United Soybean Board
Categories:Seed composition, Sustainability
Organization Project Code:1920-152-0106
Project Year:2019
Lead Principal Investigator:Felix Fritschi (University of Missouri)
Co-Principal Investigators:
Mechtihld Tegeder (Washington State University)
Keywords: protein, amino acid, transport, UPS1, AAP1, MMP1, ureide, seed composition, amino acid composition

Contributing Organizations

Funding Institutions

Information and Results

Comprehensive project details are posted online for three-years only, and final reports indefinitely. For more information on this project please contact this state soybean organization.

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Final Project Results

Updated May 16, 2020:
The overall goal of this project was to use basic information and understanding of N uptake, and ureide and amino acid transport processes to increase soybean yield and seed protein concentration, and to improve the soybean seed protein composition. As part of this project, we tested yield and seed composition of soybeans overexpressing a ureide transporter in two years at three locations in Missouri. Our analyses revealed an increase of 1 to 2% in seed protein concentration in one of three transgenic lines at two out of three locations in both years. Importantly, this increase in seed protein concentration did not come at a cost to seed yield. Additionally, we crossed the determinate ureide transporter overexpression line with an indeterminate maturity group III cultivar and developed determinate and indeterminate lines that overexpress the ureide transporter. With these lines in hand, the impact of ureide transporter overexpression can be tested in determinate and indeterminate sibling lines.

In an attempt to enhance transport of amino acids from leaves to seeds as well as the import of amino acids into seeds to increase yield, seed protein levels and protein composition, we generated soybean plants that over express new nitrogen-transport related genes. The new transgenic plants have been advanced for a couple of generations and we are on track to develop plants which stably inherit the transgene. With these plants in hand, the effect of the nitrogen-transport related genes on yield, protein content, and protein composition can be tested.

Project Years