Project Details:

Title:
High-Impact Public Research for Modified Carbohydrate Composition in U.S. Soybeans (Year 1 of 1420-532-5639)

Parent Project: This is the first year of this project.
Checkoff Organization:United Soybean Board
Categories:Breeding & genetics, Seed composition
Organization Project Code:1420-532-5639
Project Year:2014
Lead Principal Investigator:Katy M Rainey (Purdue University)
Co-Principal Investigators:
Walter Fehr (Iowa State University)
Asheesh Singh (Iowa State University)
John Brake (North Carolina State University)
James Orf (University of Minnesota)
Pengyin Chen (University of Missouri)
Henry Nguyen (University of Missouri)
Grover Shannon (University of Missouri)
Kristin Bilyeu (USDA-ARS)
Karen Hudson (USDA-ARS-Purdue University)
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Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

Composed of ten researchers in six states, a multi-disciplinary team will undertake a coordinated research effort to the advance capability to commercialize soybeans with enhanced meal value through modified carbohydrate composition. Applied and basic research activities are designed to optimize resources, prepare for commercialization of carbohydrate traits and using animal performance data, define the economic value of low oligosaccharides and high sucrose composition and other meal traits. We will develop soybean varieties in MG II-IV using existing genetic variation developed in prior funded projects, use two approaches to discover new genetic variation for carbohydrate composition, and develop germplasm specifically to define an energy value index from poultry feeding studies.

Project Objectives

1. Develop soybean germplasm and varieties with decreased oligosaccharide and elevated sucrose content, or both, in maturity groups II, III, and IV.
2. Develop soybean germplasm and varieties with modified carbohydrate composition and other valuable meal and oil composition traits.
3. Define a metabolizable energy value index for soybean based on animal performance data.

Project Deliverables

Objective 1 deliverables.
Soybean varieties in MG II - IV-S with low RFO and high sucrose from the RS2-W331 allele with yield parity to commercial varieties, both conventional and herbicide tolerant.
Chen: One conventional variety with high sucrose and low stachyose in 2015. One conventional variety with high sucrose in 2016. One RR1 variety with high sucrose and low stachyose in 2017. One RR2Y variety with high sucrose in 2018.
Fehr/Singh: All IA State varieties will include modified carbohydrate composition combined with other compositional value traits, so please refer to Objective 2 deliverables below.
Rainey: Varieties targeted are RS2-W331 in MG II-IV, conventional and RR1. 2015 initiate agronomic testing of BC5 lines, select varieties for release in 2016 or 2017.
Shannon: 2015 release one RS2-W331 MG III and one MG IV conventional soybean germplasm line with RS2-W331. 2016 release one RS2-W331 RR1 line in MG III and IV.

- Soybean germplasm with diverse compositional profiles specifically for defining meal value using poultry feeding studies.
• -New genes, alleles, and QTL modifying carbohydrate composition of soybean seeds identified.
• -Stability of carbohydrate composition defined.
• -A panel of high sucrose soybeans varieties defined and sucrose QTL mapped.
• -Impacts on seed quality and seedling vigor defined.

Objective 2 deliverables.
Soybean varieties in MG II - V with low RFO and high sucrose from the RS2-W331 allele, and high oleic acid composition.
Fehr/Singh: Producing breeder seed of two high protein conventional cultivars with Rs2 and maybe Rs3 in 2013. Producing breeder seed of three conventional cultivars with 1% linolenic acid, normal protein content, and Rs2 (maybe Rs3) the summer of 2014. Seed of the high oleic version of one line and the RR1 version of another line will be produced the summer of 2015.
Rainey: Varieties targeted are RS2-W331 in MG II-IV, high oleic, and conventional. 2015 initiate agronomic testing of BC5 lines, select varieties for release in 2016 or 2017. 2016 initiate agronomic testing of BC5 lines, select varieties for release in 2017 or 2018.
Shannon: 2016 release one group III or IV RS2-W331 conventional line with low trypsin inhibitor. 2017 release one RS2-W331 RR1 III or IV RR1 line with low trypsin inhibitor.

- Soybean germplasm with low RFOs and/or high sucrose, and higher meal protein, low trypsin inhibitor, or both.

Objective 3 deliverables.
A ME value index will be developed for each SBM relative to its control. This ME index will encompass both animal performance and digestibility data. Soybean breeders can then use this value index to develop soybean varieties.

Progress of Work

Final Project Results

Benefit to Soybean Farmers

Performance Metrics

KPI a. Phenotyping: Accurate quantification of carbohydrate composition. 3500 samples per year screened accurately and precisely.
KPI b. Genotyping markers for marker-assisted selection.
• - Gene, alleles, and QTL modifying carbohydrate composition and other genes of economic importance selected using SNP assays to improve breeding efficiency.
• - At minimum, RS2-W331 selected using M.A.S.
KPI c. Winter nurseries for genetics, breeding, and variety development. Three generations per year per breeding program used to rapidly meet project deliverables.
KPI d. Discovery of new sources of high sucrose, low RFOs, or both.
• - New genetic variation in candidate genes identified and tested for effects on seed RFO or sucrose levels, and agronomic potential.
• - New genetic variation is distributed to collaborators for breeding and animal studies, and is protected by intellectual property that promotes project objectives.
• - Valuable new genetic variation is incorporated into the breeding pipeline.
• - More valuable soybean meal carbohydrate composition is enabled.
KPI d1. Reverse genetic screening to identify mutations in candidate genes involved in carbohydrate metabolism in soybean seeds. 4000 mutagenized lines screened via reverse genetics approach for fifteen targeted candidate genes.
KPI d2. Phenotypic screening of the USDA soybean germplasm collection for sucrose and RFO composition. 500 germplasm lines screened phenotypically.

KPI Objective 1: Develop soybean germplasm varieties with decreased oligosaccharide and elevated sucrose content, or both, in relative maturity groups II, III, and IV.

KPI 1a. Variety development.
- Agronomic and compositional data collected, compiled, analyzed, interpreted, and shared.
- Varieties are released on schedule with yield parity to commodity cultivars.

KPI 1b. Germplasm development.
- Objectives and approaches adapt in response to research results from other project activities, using new information on economic value and new sources of genetic variation for carbohydrate composition.
- Target germplasm with appropriate controls in sufficient quantity are available for the ME value index studies.
- Germplasm breeding is undertaken using the best-available lines for contributing agronomic performance.
- Germplasm is shared in an open, cooperative, and timely fashion among project cooperators.

KPI 1c. Study of stability of carbohydrate composition over environments.
- Data collected from a minimum of five environments for each maturity group for stability analysis.
- Answer the question: ‘Can modified carbohydrate composition soybeans be produced stably to meet economic targets?’

KPI 1d. Define a panel of high sucrose germplasm.
- A minimum of twenty high sucrose lines identified.
- Answer the questions: ‘What is the range of sucrose composition above 8% among all soybean germplasm and which lines best represent that range?’

KPI 1e. Study impacts on seed quality.
- Emergence data collected from a minimum of five seasonal and three maternal environments.
- Answer the question: ‘Given a range of seed sources and maternal environments, does the low RFO trait reduce seed germination, seedling vigor, or field emergence potential?’

KPI 2a-2c. Develop soybean germplasm and varieties with modified carbohydrate composition and other valuable meal and oil composition traits. Please refer to KPI 1b., above.

KPI 3. Define a metabolizable energy value index for soybean based on animal performance data. A metabolizable energy (ME) value index that includes both animal performance data and digestibility data will be developed and eighteen SBM cultivars will be given such a value relative to its control. This will allow soybean breeders to develop improved breeding targets based upon improved animal feeding value.

Project Years