2014
Development of novel soybean germplasm for improving soybean for SDS resistance
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Contributor/Checkoff:
Iowa Soybean Association
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
Development of novel soybean germ plasm for improving soybean for SDS resistance
Lead Principal Investigator:
Madan Bhattacharyya, Iowa State University
Co-Principal Investigators:
Silvia Cianzio, Iowa State University
Project Code:
Contributing Organization (Checkoff):
Iowa Soybean Association
(n/a)
Institution Funded:
Iowa State University
(n/a)
Brief Project Summary:

Soybean sudden death syndrome (SDS) is a major threat to soybean production. Nationwide, the estimated soybean yield suppression from SDS in 2010 was 2.1% total yield valued at $0.82 billion; and in Iowa, the estimated financial loss was nearly $332 million. In certain years, SDS causes total crop loss in many soybean fields. It is caused by a fungal pathogen, Fusarium virguliforme.

Unique Keywords:
#breeding & genetics, #genetic resistance to diseases, #soybean bioengineering, #sudden death syndrome (sds)
Information And Results
Final Project Results

Earlier we reported mapping of the two Arabidopsis nonhost resistance genes, Pss21 and Pss25. We have also reported the identification of six candidate Pss21 genes by sequencing a bulked DNA sample of 10 susceptible families (F2:3) homozygous for the pss21 allele, and progress in sequencing of a bulked sample of 14 susceptible families (F2:3) homozygous for the pss25 allele. In this quarter, we were able to accomplish the following. We characterized T-DNA insertion mutants for each of the six candidate Pss21 genes and showed that Pss21 encodes an ABC1-like protein. Based on analyses of the sequence of the bulked sample of 14 susceptible families, we could identify only one candidate Pss25 gene. Investigation of T-DNA insertion mutants for this gene (At5g64940) established that this is the Pss25 gene because all six independent T-DNA insertion mutants for this gene are susceptible to P. sojae. Pss25 encodes a member of the BEL family of homodeodomain protein (BLH2). Currently, we are cloning Pss21 and Pss25 genes in four binary vectors for transformation of soybean.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.