Project Details:

Title:
Engineered resistance to soybean cyst nematode via induced gene silencing (RNAi)

Parent Project: Engineered resistance to soybean cyst nematode via induced gene silencing (RNAi)
Checkoff Organization:North Central Soybean Research Program
Categories:Nematodes, Breeding & genetics
Organization Project Code:
Project Year:2014
Lead Principal Investigator:Harold Trick (Kansas State University)
Co-Principal Investigators:
Jiarui Li (Kansas State University)
Tim C. Todd (Kansas State University)
John Finer (The Ohio State University)
Wayne Parrott (University of Georgia)
Lila Vodkin (University of Illinois at Urbana-Champaign)
Jack Widholm (University of Illinois-Carbondale)
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Keywords: Soybean Biotechnology, Soybean Cyst Nematode - Genetic Resistance, Virus Induced Gene Silencing (VIGS)

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

The primary goal of this research project is to establish a new set of biotech traits that have durable resistance to soybean cyst nematode (SCN). Turning off genes by a process known as RNA interference (RNAi) has tremendous potential as a new strategy to increase nematode resistance. This project will investigate the opportunities to insert target gene sequences in the SCN that will provide durable genetic materials that will be lethal to SCN populations.

Project Objectives

1. Engineer stable transgenic soybean plants with traits that can silence specific nematode genes.
2. Evaluate transgenic lines with SCN bioassays to confirm the effectiveness of the level of SCN resistance.

Project Deliverables

Progress of Work

Final Project Results

Six different nematode genes were previously identified as potential targets for gene silencing and vectors were made for soybean transformation. These genes were selected as they have the potential, if silenced in the nematode, to prevent normal egg/embryo development and/or reduce the fitness of the nematodes feeding on the transgenic soybean expressing the silencing vectors. The overall effect of expressing these silencing vectors in soybean should be enhanced resistance to SCN.

The production of transgenic lines continued this year with a number of transgenic events being recovered from five of the target genes. The sixth gene (J20) has not yielded any positive clones to date. This may be due to “off target” effects on endogenous soybean genes. To minimize this potential, Ta-si versions (an alternate gene silencing approach) of J20 and two other genes were made.

Benefit to Soybean Farmers

Performance Metrics

Project Years