This project involves studying the specific nematode populations in Nebraska soybean fields. Recently a population of nematodes with the different haplotype has been identified and is increasing in the greenhouse on standard susceptible lines. The other populations are continuing to be reared on different HG-indicator lines and standard susceptible lines to look for changes in each population's genetics. We have identified a field in northeast Nebraska with the unique haplotype. This field population is distinctively different from others with the current marker that is being used. This project expands population studies to include gene mapping and methods for rapidly identifying of HG-types...