2016
Characterization and enhancement of soybean genetic resources for soilborne disease resistance
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Contributor/Checkoff:
North Central Soybean Research Program
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
Characterization and enhancement of soybean genetic resources for soilborne disease resistance
Lead Principal Investigator:
James Kurle, University of Minnesota
Co-Principal Investigators:
Jianxin Ma, Purdue University
Katy M Rainey, Purdue University
James Orf, University of Minnesota
Nevin Young, University of Minnesota
+3 More
Project Code:
CON55573
Contributing Organization (Checkoff):
North Central Soybean Research Program
(n/a)
Institution Funded:
University of Minnesota
(n/a)
Brief Project Summary:

The research described in this proposal is the second year of a systematic approach to identification and introgression of both partial and race specific resistance into cultivars adapted to the North Central region particularly very early maturity group soybean cultivars. It is coordinated with and complements the research conducted by a USB-funded soybean disease project entitled “Genes and Markers for Resistance to Phytophthora sojae, Pythium spp., and Fusarium graminearum in Soybean”, a research team led by Dr., Anne Dorrance is working in Ohio, Missouri, Virginia, and Iowa. This collaboration expands the number of options available for managing these diseases throughout the North Central...

Unique Keywords:
#breeding & genetics, #disease resistance, #fusarium graminearum, #glycine max, #partial resistance, #phytophthora root and stem rot, #phytophthora sojae, #pythium irregulare, #pythium ultimum, #rps gene, #soybean
Information And Results
Final Project Results

Update:

View uploaded report Word file

Minnesota
During this project year tests of multiple methods of inoculation with F. graminearum were conducted to validate the methodology, effectiveness, and consistency, to be used to conduct screening for resistance to seed, seedling, and root rot caused by F. graminearum. Based on these tests, the rolled towel assay and rice inoculum method were selected as the most reliable screening methods for assessing root rot and root development.

Utilizing these methods, rolled towel assay and rice inoculum methods, we completed phenotyping and association mapping of first and second runs of screening in 280 ancestral lines for resistance or partial resistance to P. sojae and F. graminearum.

Association mapping analysis was conducted on results obtained in P. sojae and F. graminearum phenotyping indicating the location of markers for partial resistance to F. graminearum. In 280 ancestral lines two categories of marker were identified that indicated resistance to F. graminearum, one marker identified on chromosome 8 indicating resistance to root necrosis and 10 markers associated with reduced susceptibility for reduction in root mass. These markers may also be good targets for genomic selection for root rot resistance. Association mapping of phenotypic data for P.sojae partial resistance did not detect significant marker train associations for resistance to P. sojae although phenotyping indicated that 280 lines differed widely in response to P.sojae inoculation. To examine this phenomenon more closely we have elected to examine the results of biparental crosses between cultivars exhibiting a wide divergence of responses to P.sojae inoculation. Completed six crosses of lines with divergent resistance characteristics, resistant and susceptible, for use in biparental mapping of resistance and partial resistance to P. sojae.

First run consisting of one rep of evaluation of 280 lines for resistance or partial resistance to P. ultimum is underway.


Purdue:
Generate a higher resolution genetic and physical map and finer mapping of these two genes, RpsUN1 and RpsUN2. RpsUN1 gene has been narrowed from an ~1,300-kb region to an ~150.8-kb region. RpsUN2 gene was narrowed from an ~ 64-kb region down to ~35.6-kb region.

Identify possible candidate genes in the interval region by quantitative real-time PCR gene expression and further analysis of candidate genes in the RpsUN1 and RpsUN2 regions. Expression data demonstrate that only a single gene Glyma.03g034600 in the RpsUN1 region showed enhanced expression pattern after inoculation with P. sojae race 17. By contrast, three genes Glyma.16g215200, Glyma.16g215000 and Glyma.16g214900, in the RpsUN2 region showed enhanced pattern in response to P. sojae race 25. These are candidates for further validation.

Crossed and backcrossed RpsUN1 and RpsUN2 donor lines with four Purdue elite lines, and got the three backcrossing introgression lines with RpsUN1 and RpsUN2 genes, respectively.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.