Project Details:

Title:
Characterization of Phytophthora sojae and Phytophthora sansomeana populations in the North Central region & an assessment of management strategies

Parent Project: Characterization of Phytophthora sojae and Phytophthora sansomeana populations in the North Central region & an assessment of management strategies
Checkoff Organization:North Central Soybean Research Program
Categories:Soybean diseases
Organization Project Code:NCSRP -
Project Year:2018
Lead Principal Investigator:Anne Dorrance (The Ohio State University)
Co-Principal Investigators:
Alison Robertson (Iowa State University)
Martin Chilvers (Michigan State University)
Kiersten Wise (Purdue University)
Emmanuel Byamukama (South Dakota State University)
Santiago Mideros Mora (University of Illinois at Urbana-Champaign)
James Kurle (University of Minnesota)
Dean Malvick (University of Minnesota)
Loren Giesler (University of Nebraska)
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Funding Institutions

Information and Results

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Project Summary

• Each state will bait from 25 to 50 locations where P. sojae is known to occur and pathotype and genotype (SSR markers) the isolates to assess population diversity
• These data will improve our understanding of the diversity within each state enabling targeted deployment of the best resistance packages for each region
• Progress to date 6 of the 8 states were able collect soil samples during fall 2016 and spring 2017 in 85 fields. Carl Bradley in KY has received some funding for a survey and he has added 7 locations to the list for a total of more than 900 soil samples that are in currently beginning to be processed to recover isolates of P. sojae and P. sansomeana.
Seed and standard isolates have been shipped to all of the locations to serve as checks.

Project Objectives

1. Recover P. sojae from fields within each state and characterize for pathotype and genetic diversity.
2. Evaluate the new sources of resistance to these regional populations.
3. Recover P. sansomeana from fields within each state and characterize for host range, genetic diversity
4. Establish sensitivities (EC50) values for P. sojae and P. sansomeana isolates recovered from fields towards the new active ingredients ethaboxam, strobilurin, and oxathiapiprolin fungicides.
5. Comparison of new seed treatments on varieties with different resistance packages (Rps gene(s)/partial resistance) in field trials.
6. Evaluation of potential herbicide interactions with the development seed rot and stand loss.
7. Development of Crop Protection Network Outputs on the Management of Phytophthora spp. that infect soybean in the North Central and Ontario regions.

Project Deliverables

1. Recover P. sojae from fields within each state and characterize for pathotype and genetic diversity.
• Isolate collection of >2000 isolates (200 isolates per state)
• Improved understanding of diversity of pathotypes across region will inform of areas were Rps genes can and cannot be deployed
• Improved knowledge of genetic diversity across the region

2. Evaluate the new sources of resistance to these regional populations.
• Identify Rps genes that are effective for each of collaborating states
• Improved deployment of soybean varieties with Rps genes across the region will result in fewer losses due to Phytophthora root rot

3. Recover P. sansomeana from fields within each state and characterize for host range, genetic diversity.
• Isolate collection of >600 isolates (50 isolates per state)
• Improved understanding of phenotypic and genetic diversity within this species
• Improved knowledge of distribution and prevalence of the pathogen across the region
• Knowledge of effectiveness of Rps genes against pathogen

4. Establish sensitivities (EC50) values for P. sojae and P. sansomeana isolates recovered from fields towards the new active ingredients ethaboxam, strobilurin, and oxathiapiprolin fungicides.
• Establish baseline levels of fungicide sensitivity to ethaboxam and oxathiapiprolin before these active ingredients are widely used.
• Monitor sensitivity to strobilurins and detect reduced sensitivities that may have occurred in past few years based on published data (Broders et al 2007; Matthiesen et al 2016; Rojas et al, submitted) due to widespread use in seed treatments containing strobilurins.
• Information on placement of active ingredients across the region

5. Comparison of new seed treatments on varieties with different resistance packages (Rps gene(s)/partial resistance) in field trials.

6. Evaluation of potential herbicide interactions with the development seed rot and stand loss.
• Field trials will be established at least 2 locations per state during 2017 and 2018.
• Effect of different resistance packages on soybean disease and midseason PRR.
• Detect interactions between pre-emergence herbicides and watermold pathogens that increase risk of stand loss due to seedling disease
• Economic benefits/costs for the each management tactics.

7. Development of Crop Protection Network Outputs on the Management of Phytophthora spp. that infect soybean in the North Central and Ontario regions.
• Development of management guide

Progress of Work

Updated May 1, 2020:
Briefly, two meetings of subsets of the team were held to review progress, share protocols and make plans. Close to 300 fields have been sampled to monitor the presence/absence, inoculum levels, pathotype and genotype of P. sojae and P. sansomeana in the north central region.

View uploaded report Word file

View uploaded report 2 PDF file

Updated September 30, 2018:

View uploaded report Word file

View uploaded report 2 PDF file

View uploaded report 3 PDF file

Final Project Results

Updated October 31, 2018:
See attached report for final report.

Benefit to Soybean Farmers

Performance Metrics

Project Years