Updated August 28, 2020:
Results to Date
(1) We have conducted Shield Plus disinfectant for inactivation tests on Avian Reovirus (ARV) variant strains of genotypes 1 through 6, and other avian enteric viruses including Fowl Adenovirus type-1 (FAV-1), Infectious Bursa Disease Virus (IBDV), Avian Rotavirus and Herpesvirus. The Shield Plus powder product was prepared in sterile dH2O in five different concentrations of 5%, 8%, 10%, 15% and 20% for avian virus inactivation studies. Each of these avian viruses was tested separately in each concentration of the Shield Plus for 5 mi and 15 min reaction times, respectively. Thereafter, the virus-disinfectant mix was inoculated into LMH cell cultures to check if the virus was inactivated/killed or alive. Results showed that Shield Plus in 8% and above inactivated all the 6 genotypes of ARV variant strains, IBDV, FAV, avian rotavirus and herpesvirus in 5 min or 15 min reaction times.
(2) We have also tested the “Shield Plus” disinfectant for inactivation on avian respiratory viruses, including avian influenza virus (AIV), H1 through H9 AIV subtypes (all were low pathogenicity stains stored at Wiley Avian Virology Lab, Penn State University), Newcastle disease virus (NDV), and infectious bronchitis virus (IBV, the avian coronavirus). The Shield Plus powder product was prepared in the same fashion as the above for avian enteric virus tests, but the virus-disinfectant mix was inoculated into embryonating chicken eggs (ECE) to check if the virus was inactivated/killed or alive. Results showed that all these avian respiratory viruses were successfully inactivated in using the Shield Plus disinfectant in various concentrations of 2%, 5% and 10%, in 10-15 min or 30 min reaction time in room temperature.
(3) Currently, we are on preparation to test the other soft disinfectant of “Neutral Electrolyzed Water". We have obtained “Reliant Tabs", the new solid pill form of “Neutral Electrolyzed Water" or "Assist NPS’s solution, provided by the Assist Natural Products and Services LLC. We will conduct virus inactivation tests using Reliant Tabs in the next research period.
(4) In this proposed research, the most important objective is to test the efficacy of “soft” disinfectants on the ARV variants and other avian intestinal viruses in the live poultry flocks of broiler chickens and egg-laying hens. However, because the COVID-19 pandemic interference in the spring and summer semesters at Penn State University, we were unable to conduct bird trials for the virus disinfection studies in live chicken experiments during this research period. We have postponed the bird trial experiments for the next research period, and possibly we need to request an non-cost extension to complete this project.
Updated March 31, 2021:
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