Project Details:

Title:
Understanding the genetic basis of glyphosate resistance in kochia (K. scoparia)

Parent Project: This is the first year of this project.
Checkoff Organization:Kansas Soybean Commission
Categories:Weed control
Organization Project Code:
Project Year:2014
Lead Principal Investigator:Mithila Jugulam (Kansas State University)
Co-Principal Investigators:
Keywords: Glyphosate Resistant Soybean, Weed Control-Herbicide Resistance, Weed Research

Contributing Organizations

Funding Institutions

Information and Results

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Project Summary

Herbicide Resistance essentially is an inevitable consequence of the use of herbicides as a primary weed control strategy. Despite this inevitability, we must strive to delay the evolution of resistance by using herbicides in ways that are scientifically informed by evolutionary principles. A key aspect in predicting the evolutionary trajectory of a herbicide-resistance trait is to understand the genetic basis of herbicide resistance. Thus, the overall goal of the proposed research is to better predict and manage glyphosate resistance in kochia by understanding the genetic basis; specifically, the inheritance of glyphosate resistance in this weed. The outcome of this research will help determine the rate of evolution and spread of herbicide resistance in a population.

Project Objectives

1. Identify homozygous glyphosate-resistant and -susceptible accession from individually self-pollinated kochia plants.
2. Perform reciprocal crosses between homozygous glyphosate-resistant and -susceptible plants to generate F1 and F2 progeny; determine response of these progeny to glyphosate.
3. Quantify shikimate levels and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene copy number in parental, F1, and F2 progeny expressing glyphosate resistance or susceptibility.

Project Deliverables

Progress of Work

Final Project Results

The results of dose-response experiments indicate that the F1 progeny from direct as well as reciprocal crosses were resistant to glyphosate application, suggesting that the glyphosate resistance trait is transmitted via nuclear genes and there was no maternal inheritance of this trait. The F2 progeny segregated 3:1 for glyphosate resistance: susceptibility, suggesting that the glyphosate resistance trait is segregated as a single Mendelian trait. This is a two year research project and we are currently repeating inheritance of glyphosate resistance in kochia study in a different glyphosate resistant population from Kansas. Additional parental population of kochia (from Haskell County, KS) that are non-segregating for glyphosate resistance or susceptibility were identified. These plants are currently being used as parents to generate F1 progeny.

Benefit to Soybean Farmers

Performance Metrics

Project Years