Project Details:

Title:
The use of VIGS technology to decrease yield-limiting stress in soybeans

Parent Project: Development of high-throughput DNA-based gene silencing technology for soybeans
Checkoff Organization:North Central Soybean Research Program
Categories:Breeding & genetics, Soybean diseases
Organization Project Code:
Project Year:2012
Lead Principal Investigator:John Hill (Iowa State University)
Co-Principal Investigators:
Thomas Baum (Iowa State University)
Leonor Leandro (Iowa State University)
Steve Whitham (Iowa State University)
Dean Malvick (University of Minnesota)
Paul Esker (University of Wisconsin)
Craig Grau (University of Wisconsin)
Kerry Pedley (USDA/ARS-Fort Detrick, MD)
Michelle Graham (USDA/ARS-Iowa State University)
Randy Shoemaker (USDA/ARS-Iowa State University)
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Keywords: Virus-inducted Gene Silencing

Contributing Organizations

Funding Institutions

Information and Results

Comprehensive project details are posted online for three-years only, and final reports indefinitely. For more information on this project please contact this state soybean organization.

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Final Project Results

• Expanded capabilities of the VIGS system to include co-expression of two genes, marker-assisted gene silencing, and targeting any sequence regardless of reading frame.

• Distributed vector system to over 35 labs in the U.S., Korea, Germany, Canada, England, Brazil, France, and India to facilitate sharing of resources for soybean investigators.

• Have constructed ca. 750 soybean target gene sequences into THE BPMV VIGS vector.

• Archived about 500 BPMV VIGS clones in infectious soybean tissue.

• Established a library of BPMV VIGS constructs available on SoyBase.

• Identified Replicative Protein A subunit 3A as the candidate gene involved in iron deficiency chlorosis on soybean chromosome 20.

• Identified one and perhaps two genes involved in SDS resistance using the culture filtrate assay after optimization of the assay.

• Continue efforts aimed at identification of resistance genes Rpp1, Rpp3, and Rpp5 against Asian soybean rust.

• Identified a MAP kinase 4 that had a severe effect on growth and development and resulted in resistance to Soybean mosaic virus (SMV) and downy mildew. Likely functions as a positive regulator of growth and negative regulator of salicylic acid accumulation.

• Determined if and how disease determinants in a pathogen (specifically SMV in this study) change when the pathogen is subject to selective pressure conferred by a resistance gene (in this study Rsv1).

• Screened 81 VIGS constructs to identify genes that play a role in Rsv1-mediated extreme resistance to SMV infection and identified 10 VIGS constructs that compromised Rsv1-mediated resistance to provide new insight into the soybean signaling network required for extreme resistance against SMV.

• Identified interaction of HC-Pro and P3 SMV genes with host Rsv1 resistance genes. Recognition of the resistance Rsv1 gene is important for understanding resistance in soybean.

• Demonstrated VIGS occurs in soybean shoots, roots, petioles, and flowers using a soybean line expressing GFP. Determined temporal factors involved in VIGS.

• Identified two soybean lines that are optimal for use of VIGS in the white mold assay.

• Held 6-month biennial VIGS meeting to discuss results, challenges, and chart direction and progress on May 25, 2011 and October 12, 2011. Approximately 30 – 35 scientists in attendance.

Project Years