Project Details:

Title:
The use of VIGS technology to decrease yield-limiting stress in soybeans

Parent Project: Development of high-throughput DNA-based gene silencing technology for soybeans
Checkoff Organization:North Central Soybean Research Program
Categories:Breeding & genetics, Soybean diseases
Organization Project Code:
Project Year:2014
Lead Principal Investigator:John Hill (Iowa State University)
Co-Principal Investigators:
Thomas Baum (Iowa State University)
Leonor Leandro (Iowa State University)
Steve Whitham (Iowa State University)
Dean Malvick (University of Minnesota)
Craig Grau (University of Wisconsin)
Kerry Pedley (USDA/ARS-Fort Detrick, MD)
Michelle Graham (USDA/ARS-Iowa State University)
Randy Shoemaker (USDA/ARS-Iowa State University)
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Keywords: Bean Pod Mottle Virus (BPMV), Soybean Stress-Genetic Resistance, Virus-induced Gene Silencing

Contributing Organizations

Funding Institutions

Information and Results

Comprehensive project details are posted online for three-years only, and final reports indefinitely. For more information on this project please contact this state soybean organization.

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Final Project Results

The research team reports the following major accomplishments:
• They optimized high-throughput BPMV vector to support co-expression of two genes, gene silencing along with expression of a GFP marker gene to enable marker assisted gene silencing and targeting any sequence in the genome regardless of reading frame. A U.S. patent has been obtained on the process.
• The culture filtrate assay was proved more reliable than whole plant assay for identifying genes involved in SDS resistance.
• They made 120 BPMV constructs which may represent “hub” proteins to mediate resistance. Identified four “hub” genes that may be involved in Soybean mosaic virus resistance network.
• Two genes have been identified which seem to effect the SCN-soybean interaction. An attempt to silence genes at the SCN feeding site. Attempting to silence genes at the nematode feeding site.
• One “hub” (and perhaps three) gene for Rpp2-mediated resistance to Asian soybean rust has been identified. Likely two have been identified for Rpp3-mediated resistance. BAC library is being used for identification of Rsv1 locus.
• BPMV constructs RBOH-F (an NADPH oxidase) and COMT1 (Caffeic O-Methyl Transferase1) are consistently involved in genetic network for white mold resistance.
• They have generated plants transformed with transcription factors affecting yield and seed size. Increasing seed from these plants.
• Two VIGS project meetings were held to coordinate research and direction, discuss results, and analyze solutions to challenges.
• A library of VIGS constructs targeting nearly 1000 soybean genes has been developed.

Project Years