Project Details:

Discover Sources, Genes and Develop U.S. Adapted Germplasm with Improved Protein Quantity and Quality Utilizing Diversity in Cultivated and Wild soybeans (1920-152-0103-A)

Parent Project: This is the first year of this project.
Checkoff Organization:United Soybean Board
Categories:Seed composition, Breeding & genetics, Industrial use – Meal
Organization Project Code:1920-152-0103-A
Project Year:2019
Lead Principal Investigator:Rouf Mian (USDA/ARS-Ohio State University)
Co-Principal Investigators:
Benjamin Fallen (Clemson University)
Asheesh Singh (Iowa State University)
Blair Buckley (Louisiana State University)
Dechun Wang (Michigan State University)
E. Tallercio (North Carolina State University)
Leah McHale (The Ohio State University)
Leandro Mozzoni (University of Arkansas)
Zenglu Li (University of Georgia)
Aaron Lorenz (University of Minnesota)
Pengyin Chen (University of Missouri)
Henry Nguyen (University of Missouri)
Andrew Scaboo (University of Missouri)
Vince Pantalone (University of Tennessee-Institute of Agriculture)
Yong-Qiang An (USDA-ARS)
Tommy Carter (USDA-ARS)
Qijian Song (USDA-ARS)
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Keywords: protein, amino acid, seed composition, protein composition, amino acid composition, germplasm

Contributing Organizations

Funding Institutions

Information and Results

Comprehensive project details are posted online for three-years only, and final reports indefinitely. For more information on this project please contact this state soybean organization.

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Final Project Results

Updated May 1, 2020:
[Participants: Drs. Lorenz, UMN; Wang, MSU; McHale, The OSU; Singh, ISU; Chen, Nguyen, and Scaboo, Mizzou; Mozzoni, UARK; Pantalone, UT; Li, UGA; Carter, Taliercio, Song, AN, and Mian, USDA-ARS; and Fallen, Clemson Univ.]

Soybean meal accounts for 60-70% of the total value of soybean. The meal protein contents of recent commercial soybean cultivars in the U.S. have dipped below the minimum of 47.5%, needed for the national and international soybean meal markets and are reflective of a long-term pattern of slow decline in seed protein content. This is a major concern of U.S. soybean growers, who want to reverse this trend. The negative correlations of seed protein with seed yield and oil have been major constraints to the development of soybean lines with high meal protein without reduction in yield or oil. Another major concern is that U.S. varieties trace to a very narrow genetic base with low seed protein. This project aims to develop U.S. adapted soybean which has improved meal protein content, amino acid composition, and high-yield by expanding the genetic diversity. Variety development is supported by discovering new accessions, genes, and molecular markers for increased protein and essential amino acids utilizing the genetic diversity in cultivated and wild soybean. We report remarkable progress in all areas in this project in FY19, including the release of 7 high yielding cultivars/germplasm with high-meal protein and/or high genetic diversity.

Cultivar/germplasm releases: seven high yielding germplasm/cultivars with >48% meal protein and/or high genetic diversity were released/registered in FY19. Each of the lines was tested for stability of seed protein and seed yield in more than 20 environments across several states and years.

New sources of protein diversity were identified and characterized. Several high-protein lines were developed via fast neutron and characterized. Several exotic cultivated soybean lines with stable protein contents under cold and warm environments were identified and characterized. New QTL for high-protein and sulfur containing amino acids were mapped and markers for marker assisted breeding were developed.

Two separate studies indicated that seed protein contents can be increased by 2-3% using the Danbaekkong high-protein allele on chromosome 20 without compromising seed yield.

The cultivars/germplasm released by our group can be requested from the respective scientists for breeding and experimental purposes through appropriate MTAs.

Fourteen papers were published on our research results in refereed journals and many presentations were made in professional and stakeholder meetings in FY19.

Project Years